2hl9
From Proteopedia
(New page: 200px<br /><applet load="2hl9" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hl9, resolution 1.900Å" /> '''SUMO protease Ulp1 ...) |
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| - | [[Image:2hl9.gif|left|200px]]<br /><applet load="2hl9" size=" | + | [[Image:2hl9.gif|left|200px]]<br /><applet load="2hl9" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2hl9, resolution 1.900Å" /> | caption="2hl9, resolution 1.900Å" /> | ||
'''SUMO protease Ulp1 with the catalytic cysteine oxidized to a sulfonic acid'''<br /> | '''SUMO protease Ulp1 with the catalytic cysteine oxidized to a sulfonic acid'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2HL9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http:// | + | 2HL9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HL9 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:20:29 2008'' |
Revision as of 12:20, 23 January 2008
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SUMO protease Ulp1 with the catalytic cysteine oxidized to a sulfonic acid
Overview
Modification of cellular proteins by the ubiquitin-like protein SUMO is, essential for nuclear processes and cell cycle progression in yeast. The, Ulp1 protease catalyzes two essential functions in the SUMO pathway: (1), processing of full-length SUMO to its mature form and (2) deconjugation of, SUMO from targeted proteins. Selective reduction of the proteolytic, reaction produced a covalent thiohemiacetal transition state complex, between a Ulp1 C-terminal fragment and its cellular substrate Smt3, the, yeast SUMO homolog. The Ulp1-Smt3 crystal structure and functional testing, of elements within the conserved interface elucidate determinants of SUMO, recognition, processing, and deconjugation. Genetic analysis guided by the, structure further reveals a regulatory element N-terminal to the, proteolytic domain that is required for cell growth in yeast.
About this Structure
2HL9 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Ulp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeast., Mossessova E, Lima CD, Mol Cell. 2000 May;5(5):865-76. PMID:10882122
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