2hrv
From Proteopedia
(New page: 200px<br /><applet load="2hrv" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hrv, resolution 1.95Å" /> '''2A CYSTEINE PROTEINA...) |
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| - | [[Image:2hrv.jpg|left|200px]]<br /><applet load="2hrv" size=" | + | [[Image:2hrv.jpg|left|200px]]<br /><applet load="2hrv" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2hrv, resolution 1.95Å" /> | caption="2hrv, resolution 1.95Å" /> | ||
'''2A CYSTEINE PROTEINASE FROM HUMAN RHINOVIRUS 2'''<br /> | '''2A CYSTEINE PROTEINASE FROM HUMAN RHINOVIRUS 2'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of the 2A proteinase from human rhinovirus serotype | + | The crystal structure of the 2A proteinase from human rhinovirus serotype 2 (HRV2-2A(pro)) has been solved to 1.95 A resolution. The structure has an unusual, although chymotrypsin-related, fold comprising a unique four-stranded beta sheet as the N-terminal domain and a six-stranded beta barrel as the C-terminal domain. A tightly bound zinc ion, essential for the stability of HRV2-2A(pro), is tetrahedrally coordinated by three cysteine sulfurs and one histidine nitrogen. The active site consists of a catalytic triad formed by His18, Asp35 and Cys106. Asp35 is additionally involved in an extensive hydrogen-bonding network. Modelling studies reveal a substrate-induced fit that explains the specificity of the subsites S4, S2, S1 and S1'. The structure of HRV2-2A(pro) suggests the mechanism of the cis cleavage and its release from the polyprotein. |
==About this Structure== | ==About this Structure== | ||
| - | 2HRV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_rhinovirus_2 Human rhinovirus 2] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Picornain_2A Picornain 2A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.29 3.4.22.29] Full crystallographic information is available from [http:// | + | 2HRV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_rhinovirus_2 Human rhinovirus 2] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Picornain_2A Picornain 2A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.29 3.4.22.29] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HRV OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Picornain 2A]] | [[Category: Picornain 2A]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Cherney, M | + | [[Category: Cherney, M M.]] |
| - | [[Category: James, M | + | [[Category: James, M N.G.]] |
[[Category: Kuechler, E.]] | [[Category: Kuechler, E.]] | ||
| - | [[Category: Liebig, H | + | [[Category: Liebig, H D.]] |
| - | [[Category: Petersen, J | + | [[Category: Petersen, J F.W.]] |
[[Category: Skern, T.]] | [[Category: Skern, T.]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: hydrolase (cysteine proteinase)]] | [[Category: hydrolase (cysteine proteinase)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:45:03 2008'' |
Revision as of 15:45, 21 February 2008
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2A CYSTEINE PROTEINASE FROM HUMAN RHINOVIRUS 2
Overview
The crystal structure of the 2A proteinase from human rhinovirus serotype 2 (HRV2-2A(pro)) has been solved to 1.95 A resolution. The structure has an unusual, although chymotrypsin-related, fold comprising a unique four-stranded beta sheet as the N-terminal domain and a six-stranded beta barrel as the C-terminal domain. A tightly bound zinc ion, essential for the stability of HRV2-2A(pro), is tetrahedrally coordinated by three cysteine sulfurs and one histidine nitrogen. The active site consists of a catalytic triad formed by His18, Asp35 and Cys106. Asp35 is additionally involved in an extensive hydrogen-bonding network. Modelling studies reveal a substrate-induced fit that explains the specificity of the subsites S4, S2, S1 and S1'. The structure of HRV2-2A(pro) suggests the mechanism of the cis cleavage and its release from the polyprotein.
About this Structure
2HRV is a Single protein structure of sequence from Human rhinovirus 2 with as ligand. Active as Picornain 2A, with EC number 3.4.22.29 Full crystallographic information is available from OCA.
Reference
The structure of the 2A proteinase from a common cold virus: a proteinase responsible for the shut-off of host-cell protein synthesis., Petersen JF, Cherney MM, Liebig HD, Skern T, Kuechler E, James MN, EMBO J. 1999 Oct 15;18(20):5463-75. PMID:10523291
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