2i13
From Proteopedia
(New page: 200px<br /><applet load="2i13" size="450" color="white" frame="true" align="right" spinBox="true" caption="2i13, resolution 1.960Å" /> '''Aart, a six finger ...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2i13.gif|left|200px]]<br /><applet load="2i13" size=" | + | [[Image:2i13.gif|left|200px]]<br /><applet load="2i13" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2i13, resolution 1.960Å" /> | caption="2i13, resolution 1.960Å" /> | ||
'''Aart, a six finger zinc finger designed to recognize ANN triplets'''<br /> | '''Aart, a six finger zinc finger designed to recognize ANN triplets'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Cys2-His2 zinc fingers are one of the most common types of DNA-binding | + | Cys2-His2 zinc fingers are one of the most common types of DNA-binding domains. Modifications to zinc-finger binding specificity have recently enabled custom DNA-binding proteins to be designed to a wide array of target sequences. We present here a 1.96 A structure of Aart, a designed six-zinc finger protein, bound to a consensus DNA target site. This is the first structure of a designed protein with six fingers, and was intended to provide insights into the unusual affinity and specificity characteristics of this protein. Most protein-DNA contacts were found to be consistent with expectations, while others were unanticipated or insufficient to explain specificity. Several were unexpectedly mediated by glycerol, water molecules or amino acid-base stacking interactions. These results challenge some conventional concepts of recognition, particularly the finding that triplets containing 5'A, C, or T are typically not specified by direct interaction with the amino acid in position 6 of the recognition helix. |
==About this Structure== | ==About this Structure== | ||
| - | 2I13 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with ZN and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2I13 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I13 OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bhakta, M.]] | [[Category: Bhakta, M.]] | ||
| - | [[Category: Crotty, J | + | [[Category: Crotty, J W.]] |
| - | [[Category: Horton, N | + | [[Category: Horton, N C.]] |
| - | [[Category: III, C | + | [[Category: III, C F.Barbas.]] |
| - | [[Category: Segal, D | + | [[Category: Segal, D J.]] |
[[Category: GOL]] | [[Category: GOL]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
| Line 23: | Line 23: | ||
[[Category: zinc finger]] | [[Category: zinc finger]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:47:55 2008'' |
Revision as of 15:47, 21 February 2008
|
Aart, a six finger zinc finger designed to recognize ANN triplets
Overview
Cys2-His2 zinc fingers are one of the most common types of DNA-binding domains. Modifications to zinc-finger binding specificity have recently enabled custom DNA-binding proteins to be designed to a wide array of target sequences. We present here a 1.96 A structure of Aart, a designed six-zinc finger protein, bound to a consensus DNA target site. This is the first structure of a designed protein with six fingers, and was intended to provide insights into the unusual affinity and specificity characteristics of this protein. Most protein-DNA contacts were found to be consistent with expectations, while others were unanticipated or insufficient to explain specificity. Several were unexpectedly mediated by glycerol, water molecules or amino acid-base stacking interactions. These results challenge some conventional concepts of recognition, particularly the finding that triplets containing 5'A, C, or T are typically not specified by direct interaction with the amino acid in position 6 of the recognition helix.
About this Structure
2I13 is a Single protein structure of sequence from Mus musculus with and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of Aart, a designed six-finger zinc finger peptide, bound to DNA., Segal DJ, Crotty JW, Bhakta MS, Barbas CF 3rd, Horton NC, J Mol Biol. 2006 Oct 20;363(2):405-21. Epub 2006 Aug 11. PMID:16963084
Page seeded by OCA on Thu Feb 21 17:47:55 2008
Categories: Mus musculus | Single protein | Bhakta, M. | Crotty, J W. | Horton, N C. | III, C F.Barbas. | Segal, D J. | GOL | ZN | Dna binding | Zinc finger
