2i6t
From Proteopedia
(New page: 200px<br /><applet load="2i6t" size="450" color="white" frame="true" align="right" spinBox="true" caption="2i6t, resolution 2.10Å" /> '''Orthorhombic Structu...) |
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| - | [[Image:2i6t.jpg|left|200px]]<br /><applet load="2i6t" size=" | + | [[Image:2i6t.jpg|left|200px]]<br /><applet load="2i6t" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2i6t, resolution 2.10Å" /> | caption="2i6t, resolution 2.10Å" /> | ||
'''Orthorhombic Structure of the LDH domain of Human Ubiquitin-conjugating Enzyme E2-like Isoform A'''<br /> | '''Orthorhombic Structure of the LDH domain of Human Ubiquitin-conjugating Enzyme E2-like Isoform A'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Recent studies have shown that ubiquitination is an essential factor in | + | Recent studies have shown that ubiquitination is an essential factor in endosomal sorting and virus assembly. The human TSG101 gene has been demonstrated to belong to a group of genes coding for apparently inactive E2 ubiquitin-conjugating enzymes, which exert regulatory effects on E2 activity in cellular ubiquitination processes. In this study, a novel human cDNA (UEV3) encoding a putative protein of 379 amino acids was isolated from a human placenta library that may represent a partial paralogue of human TSG101. The predicted protein contains an N-terminal domain homologous to the catalytic domain of ubiquitin-conjugating enzymes (Ubc), which is fused to a sequence showing significant homology to members of the lactate dehydrogenase protein family. The UEV3 gene is located on chromosome 11 closely adjacent to TSG101 and LDH-C. Northern blot and UEV3-specific reverse transcription/polymerase chain reaction (RT/PCR) analyses of various colon carcinoma cell lines as well as both normal and tumor samples from colon revealed an expression of the UEV3 cDNA in all tested samples. |
==About this Structure== | ==About this Structure== | ||
| - | 2I6T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2I6T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I6T OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Arrowsmith, C | + | [[Category: Arrowsmith, C H.]] |
| - | [[Category: Avvakumov, G | + | [[Category: Avvakumov, G V.]] |
[[Category: Bochkarev, A.]] | [[Category: Bochkarev, A.]] | ||
[[Category: Butler-Cole, C.]] | [[Category: Butler-Cole, C.]] | ||
[[Category: Dhe-Paganon, S.]] | [[Category: Dhe-Paganon, S.]] | ||
| - | [[Category: Edwards, A | + | [[Category: Edwards, A M.]] |
| - | [[Category: Jr., P | + | [[Category: Jr., P J.Finerty.]] |
| - | [[Category: Newman, E | + | [[Category: Newman, E M.]] |
[[Category: Sundstrom, M.]] | [[Category: Sundstrom, M.]] | ||
[[Category: Tempel, W.]] | [[Category: Tempel, W.]] | ||
| - | [[Category: Walker, J | + | [[Category: Walker, J R.]] |
[[Category: Weigelt, J.]] | [[Category: Weigelt, J.]] | ||
[[Category: Xue, S.]] | [[Category: Xue, S.]] | ||
| Line 32: | Line 32: | ||
[[Category: ubiquitin-protein ligase]] | [[Category: ubiquitin-protein ligase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:49:41 2008'' |
Revision as of 15:49, 21 February 2008
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Orthorhombic Structure of the LDH domain of Human Ubiquitin-conjugating Enzyme E2-like Isoform A
Overview
Recent studies have shown that ubiquitination is an essential factor in endosomal sorting and virus assembly. The human TSG101 gene has been demonstrated to belong to a group of genes coding for apparently inactive E2 ubiquitin-conjugating enzymes, which exert regulatory effects on E2 activity in cellular ubiquitination processes. In this study, a novel human cDNA (UEV3) encoding a putative protein of 379 amino acids was isolated from a human placenta library that may represent a partial paralogue of human TSG101. The predicted protein contains an N-terminal domain homologous to the catalytic domain of ubiquitin-conjugating enzymes (Ubc), which is fused to a sequence showing significant homology to members of the lactate dehydrogenase protein family. The UEV3 gene is located on chromosome 11 closely adjacent to TSG101 and LDH-C. Northern blot and UEV3-specific reverse transcription/polymerase chain reaction (RT/PCR) analyses of various colon carcinoma cell lines as well as both normal and tumor samples from colon revealed an expression of the UEV3 cDNA in all tested samples.
About this Structure
2I6T is a Single protein structure of sequence from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.
Reference
Identification and characterization of UEV3, a human cDNA with similarities to inactive E2 ubiquitin-conjugating enzymes., Kloor M, Bork P, Duwe A, Klaes R, von Knebel Doeberitz M, Ridder R, Biochim Biophys Acta. 2002 Dec 12;1579(2-3):219-24. PMID:12427560
Page seeded by OCA on Thu Feb 21 17:49:41 2008
Categories: Homo sapiens | Single protein | Arrowsmith, C H. | Avvakumov, G V. | Bochkarev, A. | Butler-Cole, C. | Dhe-Paganon, S. | Edwards, A M. | Jr., P J.Finerty. | Newman, E M. | Sundstrom, M. | Tempel, W. | Walker, J R. | Weigelt, J. | Xue, S. | GOL | SO4 | L-lactate dehydrogenase | Oxidoreductase | Ubiquitin-protein ligase
