2ihs
From Proteopedia
(New page: 200px<br /><applet load="2ihs" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ihs, resolution 2.2Å" /> '''Crystal structure of ...) |
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| - | [[Image:2ihs.jpg|left|200px]]<br /><applet load="2ihs" size=" | + | [[Image:2ihs.jpg|left|200px]]<br /><applet load="2ihs" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2ihs, resolution 2.2Å" /> | caption="2ihs, resolution 2.2Å" /> | ||
'''Crystal structure of the B30.2/SPRY domain of GUSTAVUS in complex with a 20-residue VASA peptide'''<br /> | '''Crystal structure of the B30.2/SPRY domain of GUSTAVUS in complex with a 20-residue VASA peptide'''<br /> | ||
==Overview== | ==Overview== | ||
| - | B30.2/SPRY domains are found in numerous proteins that cover a wide | + | B30.2/SPRY domains are found in numerous proteins that cover a wide spectrum of biological functions, including regulation of cytokine signaling and innate retroviral restriction. Herein, we report the crystal structure of the B30.2/SPRY domain of a SPRY domain-containing SOCS box (SSB) protein, GUSTAVUS, complexed with a 20 amino acid peptide derived from the RNA helicase VASA, revealing how these domains recognize target proteins. The peptide-binding site is conformationally rigid and has a preformed pocket. The interaction between the pocket and the Asp-Ile-Asn-Asn-Asn-Asn sequence within the peptide accounts for the high-affinity binding between GUSTAVUS and VASA. This observation led to a facile identification of the Glu-Leu-Asn-Asn-Asn-Leu sequence as the recognition motif in a proapoptotic protein Par-4 for its interaction with a GUSTAVUS homolog, SSB-1. Ensuing analyses indicated that many B30.2/SPRY domains have a similar preformed pocket, which would allow them to bind multiple targets. |
==About this Structure== | ==About this Structure== | ||
| - | 2IHS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http:// | + | 2IHS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IHS OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Oh, B | + | [[Category: Oh, B H.]] |
| - | [[Category: Park, S | + | [[Category: Park, S Y.]] |
| - | [[Category: Woo, J | + | [[Category: Woo, J S.]] |
| - | [[Category: b30 | + | [[Category: b30 2/spry]] |
[[Category: f-box-spry]] | [[Category: f-box-spry]] | ||
[[Category: gustavus]] | [[Category: gustavus]] | ||
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[[Category: vasa]] | [[Category: vasa]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:52:50 2008'' |
Revision as of 15:52, 21 February 2008
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Crystal structure of the B30.2/SPRY domain of GUSTAVUS in complex with a 20-residue VASA peptide
Overview
B30.2/SPRY domains are found in numerous proteins that cover a wide spectrum of biological functions, including regulation of cytokine signaling and innate retroviral restriction. Herein, we report the crystal structure of the B30.2/SPRY domain of a SPRY domain-containing SOCS box (SSB) protein, GUSTAVUS, complexed with a 20 amino acid peptide derived from the RNA helicase VASA, revealing how these domains recognize target proteins. The peptide-binding site is conformationally rigid and has a preformed pocket. The interaction between the pocket and the Asp-Ile-Asn-Asn-Asn-Asn sequence within the peptide accounts for the high-affinity binding between GUSTAVUS and VASA. This observation led to a facile identification of the Glu-Leu-Asn-Asn-Asn-Leu sequence as the recognition motif in a proapoptotic protein Par-4 for its interaction with a GUSTAVUS homolog, SSB-1. Ensuing analyses indicated that many B30.2/SPRY domains have a similar preformed pocket, which would allow them to bind multiple targets.
About this Structure
2IHS is a Protein complex structure of sequences from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Structural basis for protein recognition by B30.2/SPRY domains., Woo JS, Suh HY, Park SY, Oh BH, Mol Cell. 2006 Dec 28;24(6):967-76. PMID:17189197
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