2iob
From Proteopedia
(New page: 200px<br /><applet load="2iob" size="450" color="white" frame="true" align="right" spinBox="true" caption="2iob, resolution 2.2Å" /> '''E. coli Bifunctional ...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2iob.jpg|left|200px]]<br /><applet load="2iob" size=" | + | [[Image:2iob.jpg|left|200px]]<br /><applet load="2iob" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2iob, resolution 2.2Å" /> | caption="2iob, resolution 2.2Å" /> | ||
'''E. coli Bifunctional glutathionylspermidine synthetase/amidase Apo protein'''<br /> | '''E. coli Bifunctional glutathionylspermidine synthetase/amidase Apo protein'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Most organisms use glutathione to regulate intracellular thiol redox | + | Most organisms use glutathione to regulate intracellular thiol redox balance and protect against oxidative stress; protozoa, however, utilize trypanothione for this purpose. Trypanothione biosynthesis requires ATP-dependent conjugation of glutathione (GSH) to the two terminal amino groups of spermidine by glutathionylspermidine synthetase (GspS) and trypanothione synthetase (TryS), which are considered as drug targets. GspS catalyzes the penultimate step of the biosynthesis-amide bond formation between spermidine and the glycine carboxylate of GSH. We report herein five crystal structures of Escherichia coli GspS in complex with substrate, product or inhibitor. The C-terminal of GspS belongs to the ATP-grasp superfamily with a similar fold to the human glutathione synthetase. GSH is likely phosphorylated at one of two GSH-binding sites to form an acylphosphate intermediate that then translocates to the other site for subsequent nucleophilic addition of spermidine. We also identify essential amino acids involved in the catalysis. Our results constitute the first structural information on the biochemical features of parasite homologs (including TryS) that underlie their broad specificity for polyamines. |
==About this Structure== | ==About this Structure== | ||
| - | 2IOB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 2IOB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IOB OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Chiang, B | + | [[Category: Chiang, B Y.]] |
| - | [[Category: Chong, C | + | [[Category: Chong, C M.]] |
| - | [[Category: Chou, C | + | [[Category: Chou, C C.]] |
| - | [[Category: Coward, J | + | [[Category: Coward, J K.]] |
| - | [[Category: Ko, T | + | [[Category: Ko, T P.]] |
| - | [[Category: Lin, C | + | [[Category: Lin, C H.]] |
| - | [[Category: Pai, C | + | [[Category: Pai, C H.]] |
| - | [[Category: Wang, A | + | [[Category: Wang, A H.J.]] |
| - | [[Category: Yen, F | + | [[Category: Yen, F J.]] |
[[Category: bifunctional glutathionylspermidine synthetase/amidase]] | [[Category: bifunctional glutathionylspermidine synthetase/amidase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:54:39 2008'' |
Revision as of 15:54, 21 February 2008
|
E. coli Bifunctional glutathionylspermidine synthetase/amidase Apo protein
Overview
Most organisms use glutathione to regulate intracellular thiol redox balance and protect against oxidative stress; protozoa, however, utilize trypanothione for this purpose. Trypanothione biosynthesis requires ATP-dependent conjugation of glutathione (GSH) to the two terminal amino groups of spermidine by glutathionylspermidine synthetase (GspS) and trypanothione synthetase (TryS), which are considered as drug targets. GspS catalyzes the penultimate step of the biosynthesis-amide bond formation between spermidine and the glycine carboxylate of GSH. We report herein five crystal structures of Escherichia coli GspS in complex with substrate, product or inhibitor. The C-terminal of GspS belongs to the ATP-grasp superfamily with a similar fold to the human glutathione synthetase. GSH is likely phosphorylated at one of two GSH-binding sites to form an acylphosphate intermediate that then translocates to the other site for subsequent nucleophilic addition of spermidine. We also identify essential amino acids involved in the catalysis. Our results constitute the first structural information on the biochemical features of parasite homologs (including TryS) that underlie their broad specificity for polyamines.
About this Structure
2IOB is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Dual binding sites for translocation catalysis by Escherichia coli glutathionylspermidine synthetase., Pai CH, Chiang BY, Ko TP, Chou CC, Chong CM, Yen FJ, Chen S, Coward JK, Wang AH, Lin CH, EMBO J. 2006 Dec 13;25(24):5970-82. Epub 2006 Nov 23. PMID:17124497
Page seeded by OCA on Thu Feb 21 17:54:39 2008
