2ip1
From Proteopedia
(New page: 200px<br /><applet load="2ip1" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ip1, resolution 1.80Å" /> '''Crystal Structure An...) |
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| - | [[Image:2ip1.gif|left|200px]]<br /><applet load="2ip1" size=" | + | [[Image:2ip1.gif|left|200px]]<br /><applet load="2ip1" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2ip1, resolution 1.80Å" /> | caption="2ip1, resolution 1.80Å" /> | ||
'''Crystal Structure Analysis of S. cerevisiae Tryptophanyl tRNA Synthetase'''<br /> | '''Crystal Structure Analysis of S. cerevisiae Tryptophanyl tRNA Synthetase'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2IP1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with PG4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Tryptophan--tRNA_ligase Tryptophan--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.2 6.1.1.2] Full crystallographic information is available from [http:// | + | 2IP1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=PG4:'>PG4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Tryptophan--tRNA_ligase Tryptophan--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.2 6.1.1.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IP1 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:45:15 2008'' |
Revision as of 12:45, 23 January 2008
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Crystal Structure Analysis of S. cerevisiae Tryptophanyl tRNA Synthetase
Overview
Saccharomyces cerevisiae is an ideal host from which to obtain high levels, of posttranslationally modified eukaryotic proteins for x-ray, crystallography. However, extensive replacement of methionine by, selenomethionine for anomalous dispersion phasing has proven intractable, in yeast. We report a general method to incorporate selenomethionine into, proteins expressed in yeast based on manipulation of the appropriate, metabolic pathways. sam1(-) sam2(-) mutants, in which the conversion of, methionine to S-adenosylmethionine is blocked, exhibit reduced, selenomethionine toxicity compared with wild-type yeast, increased, production of protein during growth in selenomethionine, and efficient, replacement of methionine by selenomethionine, based on quantitative mass, spectrometry and x-ray crystallography. The structure of yeast, tryptophanyl-tRNA synthetase was solved to 1.8 A by using multiwavelength, anomalous dispersion phasing with protein that was expressed and purified, from the sam1(-) sam2(-) strain grown in selenomethionine. Six of eight, selenium residues were located in the structure.
About this Structure
2IP1 is a Single protein structure of sequence from Saccharomyces cerevisiae with as ligand. Active as Tryptophan--tRNA ligase, with EC number 6.1.1.2 Full crystallographic information is available from OCA.
Reference
Blocking S-adenosylmethionine synthesis in yeast allows selenomethionine incorporation and multiwavelength anomalous dispersion phasing., Malkowski MG, Quartley E, Friedman AE, Babulski J, Kon Y, Wolfley J, Said M, Luft JR, Phizicky EM, DeTitta GT, Grayhack EJ, Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6678-83. Epub 2007 Apr 10. PMID:17426150
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