2izy
From Proteopedia
(New page: 200px<br /><applet load="2izy" size="450" color="white" frame="true" align="right" spinBox="true" caption="2izy, resolution 2.20Å" /> '''MOLECULAR BASIS OF A...) |
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| - | [[Image:2izy.jpg|left|200px]]<br /><applet load="2izy" size=" | + | [[Image:2izy.jpg|left|200px]]<br /><applet load="2izy" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2izy, resolution 2.20Å" /> | caption="2izy, resolution 2.20Å" /> | ||
'''MOLECULAR BASIS OF AKAP SPECIFICITY FOR PKA REGULATORY SUBUNITS'''<br /> | '''MOLECULAR BASIS OF AKAP SPECIFICITY FOR PKA REGULATORY SUBUNITS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Localization of cyclic AMP (cAMP)-dependent protein kinase (PKA) by A | + | Localization of cyclic AMP (cAMP)-dependent protein kinase (PKA) by A kinase-anchoring proteins (AKAPs) restricts the action of this broad specificity kinase. The high-resolution crystal structures of the docking and dimerization (D/D) domain of the RIIalpha regulatory subunit of PKA both in the apo state and in complex with the high-affinity anchoring peptide AKAP-IS explain the molecular basis for AKAP-regulatory subunit recognition. AKAP-IS folds into an amphipathic alpha helix that engages an essentially preformed shallow groove on the surface of the RII dimer D/D domains. Conserved AKAP aliphatic residues dominate interactions to RII at the predominantly hydrophobic interface, whereas polar residues are important in conferring R subunit isoform specificity. Using a peptide screening approach, we have developed SuperAKAP-IS, a peptide that is 10,000-fold more selective for the RII isoform relative to RI and can be used to assess the impact of PKA isoform-selective anchoring on cAMP-responsive events inside cells. |
==About this Structure== | ==About this Structure== | ||
| - | 2IZY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Active as [http://en.wikipedia.org/wiki/cAMP-dependent_protein_kinase cAMP-dependent protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.11 2.7.11.11] Full crystallographic information is available from [http:// | + | 2IZY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Active as [http://en.wikipedia.org/wiki/cAMP-dependent_protein_kinase cAMP-dependent protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.11 2.7.11.11] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IZY OCA]. |
==Reference== | ==Reference== | ||
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[[Category: cAMP-dependent protein kinase]] | [[Category: cAMP-dependent protein kinase]] | ||
[[Category: Barford, D.]] | [[Category: Barford, D.]] | ||
| - | [[Category: Carlson, C | + | [[Category: Carlson, C R.]] |
[[Category: Dokurno, P.]] | [[Category: Dokurno, P.]] | ||
| - | [[Category: Gold, M | + | [[Category: Gold, M G.]] |
[[Category: Hoshi, N.]] | [[Category: Hoshi, N.]] | ||
[[Category: Lygren, B.]] | [[Category: Lygren, B.]] | ||
[[Category: Mcconnachie, G.]] | [[Category: Mcconnachie, G.]] | ||
| - | [[Category: Scott, J | + | [[Category: Scott, J D.]] |
[[Category: Tasken, K.]] | [[Category: Tasken, K.]] | ||
[[Category: acetylation]] | [[Category: acetylation]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:57:50 2008'' |
Revision as of 15:57, 21 February 2008
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MOLECULAR BASIS OF AKAP SPECIFICITY FOR PKA REGULATORY SUBUNITS
Overview
Localization of cyclic AMP (cAMP)-dependent protein kinase (PKA) by A kinase-anchoring proteins (AKAPs) restricts the action of this broad specificity kinase. The high-resolution crystal structures of the docking and dimerization (D/D) domain of the RIIalpha regulatory subunit of PKA both in the apo state and in complex with the high-affinity anchoring peptide AKAP-IS explain the molecular basis for AKAP-regulatory subunit recognition. AKAP-IS folds into an amphipathic alpha helix that engages an essentially preformed shallow groove on the surface of the RII dimer D/D domains. Conserved AKAP aliphatic residues dominate interactions to RII at the predominantly hydrophobic interface, whereas polar residues are important in conferring R subunit isoform specificity. Using a peptide screening approach, we have developed SuperAKAP-IS, a peptide that is 10,000-fold more selective for the RII isoform relative to RI and can be used to assess the impact of PKA isoform-selective anchoring on cAMP-responsive events inside cells.
About this Structure
2IZY is a Single protein structure of sequence from Mus musculus. Active as cAMP-dependent protein kinase, with EC number 2.7.11.11 Full crystallographic information is available from OCA.
Reference
Molecular basis of AKAP specificity for PKA regulatory subunits., Gold MG, Lygren B, Dokurno P, Hoshi N, McConnachie G, Tasken K, Carlson CR, Scott JD, Barford D, Mol Cell. 2006 Nov 3;24(3):383-95. PMID:17081989
Page seeded by OCA on Thu Feb 21 17:57:50 2008
Categories: Mus musculus | Single protein | CAMP-dependent protein kinase | Barford, D. | Carlson, C R. | Dokurno, P. | Gold, M G. | Hoshi, N. | Lygren, B. | Mcconnachie, G. | Scott, J D. | Tasken, K. | Acetylation | Camp | Camp-binding | D/d | Kinase | Nucleotide-binding | Phosphorylation | Pka | Rii | Transferase
