2nmv
From Proteopedia
(New page: 200px<br /><applet load="2nmv" size="450" color="white" frame="true" align="right" spinBox="true" caption="2nmv, resolution 2.95Å" /> '''Damage detection by ...) |
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| - | [[Image:2nmv.gif|left|200px]]<br /><applet load="2nmv" size=" | + | [[Image:2nmv.gif|left|200px]]<br /><applet load="2nmv" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2nmv, resolution 2.95Å" /> | caption="2nmv, resolution 2.95Å" /> | ||
'''Damage detection by the UvrABC pathway: Crystal structure of UvrB bound to fluorescein-adducted DNA'''<br /> | '''Damage detection by the UvrABC pathway: Crystal structure of UvrB bound to fluorescein-adducted DNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | UvrB is the damage recognition element of the highly conserved UvrABC | + | UvrB is the damage recognition element of the highly conserved UvrABC pathway that functions in the removal of bulky DNA adducts. Pivotal to this is the formation of a damage detection complex that relies on the ability of UvrB to locate and sequester diverse lesions. Whilst structures of UvrB bound to DNA have recently been reported, none address the issue of lesion recognition. Here, we describe the crystal structure of UvrB bound to a pentanucleotide containing a single fluorescein-adducted thymine that reveals a unique mechanism for damage detection entirely dependent on the exclusion of lesions larger than an undamaged nucleotide. |
==About this Structure== | ==About this Structure== | ||
| - | 2NMV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with ADP, NML and FLU as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2NMV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=ADP:'>ADP</scene>, <scene name='pdbligand=NML:'>NML</scene> and <scene name='pdbligand=FLU:'>FLU</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NMV OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Barrett, T | + | [[Category: Barrett, T E.]] |
[[Category: Eryilmaz, J.]] | [[Category: Eryilmaz, J.]] | ||
[[Category: Geddes, S.]] | [[Category: Geddes, S.]] | ||
| - | [[Category: Waters, T | + | [[Category: Waters, T R.]] |
[[Category: ADP]] | [[Category: ADP]] | ||
[[Category: FLU]] | [[Category: FLU]] | ||
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[[Category: t-fluorescein]] | [[Category: t-fluorescein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:08:31 2008'' |
Revision as of 16:08, 21 February 2008
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Damage detection by the UvrABC pathway: Crystal structure of UvrB bound to fluorescein-adducted DNA
Overview
UvrB is the damage recognition element of the highly conserved UvrABC pathway that functions in the removal of bulky DNA adducts. Pivotal to this is the formation of a damage detection complex that relies on the ability of UvrB to locate and sequester diverse lesions. Whilst structures of UvrB bound to DNA have recently been reported, none address the issue of lesion recognition. Here, we describe the crystal structure of UvrB bound to a pentanucleotide containing a single fluorescein-adducted thymine that reveals a unique mechanism for damage detection entirely dependent on the exclusion of lesions larger than an undamaged nucleotide.
About this Structure
2NMV is a Protein complex structure of sequences from Bacillus subtilis with , and as ligands. Full crystallographic information is available from OCA.
Reference
Damage detection by the UvrABC pathway: crystal structure of UvrB bound to fluorescein-adducted DNA., Waters TR, Eryilmaz J, Geddes S, Barrett TE, FEBS Lett. 2006 Nov 27;580(27):6423-7. Epub 2006 Nov 3. PMID:17097086
Page seeded by OCA on Thu Feb 21 18:08:31 2008
Categories: Bacillus subtilis | Protein complex | Barrett, T E. | Eryilmaz, J. | Geddes, S. | Waters, T R. | ADP | FLU | NML | Hairpin | Protein-dna complex | T-fluorescein
