1ckm
From Proteopedia
(New page: 200px<br /> <applet load="1ckm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ckm, resolution 2.5Å" /> '''STRUCTURE OF TWO DIF...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1CKM is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1]] with GTP as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.50 2.7.7.50]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CKM OCA]]. | + | 1CKM is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1]] with GTP as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/mRNA_guanylyltransferase mRNA guanylyltransferase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.50 2.7.7.50]]. Structure known Active Sites: GPA and GPB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CKM OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: Paramecium bursaria chlorella virus 1]] | [[Category: Paramecium bursaria chlorella virus 1]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| + | [[Category: mRNA guanylyltransferase]] | ||
[[Category: Doherty, A.J.]] | [[Category: Doherty, A.J.]] | ||
[[Category: Hakansson, K.]] | [[Category: Hakansson, K.]] | ||
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[[Category: nucleotidyltransferase]] | [[Category: nucleotidyltransferase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:36:13 2007'' |
Revision as of 12:31, 30 October 2007
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STRUCTURE OF TWO DIFFERENT CONFORMATIONS OF MRNA CAPPING ENZYME IN COMPLEX WITH GTP
Overview
We have solved the crystal structure of an mRNA capping enzyme at 2.5 A, resolution. The enzyme comprises two domains with a deep, but narrow, cleft between them. The two molecules in the crystallographic asymmetric, unit adopt very different conformations; both contain a bound GTP, but one, protein molecule is in an open conformation while the other is in a closed, conformation. Only in the closed conformation is the enzyme able to bind, manganese ions and undergo catalysis within the crystals to yield the, covalent guanylated enzyme intermediate. These structures provide direct, evidence for a mechanism that involves a significant conformational change, in the enzyme during catalysis.
About this Structure
1CKM is a [Single protein] structure of sequence from [Paramecium bursaria chlorella virus 1] with GTP as [ligand]. Active as [mRNA guanylyltransferase], with EC number [2.7.7.50]. Structure known Active Sites: GPA and GPB. Full crystallographic information is available from [OCA].
Reference
X-ray crystallography reveals a large conformational change during guanyl transfer by mRNA capping enzymes., Hakansson K, Doherty AJ, Shuman S, Wigley DB, Cell. 1997 May 16;89(4):545-53. PMID:9160746
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