2oaj
From Proteopedia
(New page: 200px<br /><applet load="2oaj" size="450" color="white" frame="true" align="right" spinBox="true" caption="2oaj, resolution 2.40Å" /> '''Crystal structure of...) |
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| - | [[Image:2oaj.jpg|left|200px]]<br /><applet load="2oaj" size=" | + | [[Image:2oaj.jpg|left|200px]]<br /><applet load="2oaj" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2oaj, resolution 2.40Å" /> | caption="2oaj, resolution 2.40Å" /> | ||
'''Crystal structure of Sro7 from S. cerevisiae'''<br /> | '''Crystal structure of Sro7 from S. cerevisiae'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2OAJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with ZN and NA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2OAJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OAJ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: wd40 repeat]] | [[Category: wd40 repeat]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:03:07 2008'' |
Revision as of 10:03, 23 January 2008
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Crystal structure of Sro7 from S. cerevisiae
Overview
Polarized exocytosis requires coordination between the actin cytoskeleton, and the exocytic machinery responsible for fusion of secretory vesicles at, specific sites on the plasma membrane. Fusion requires formation of a, complex between a vesicle-bound R-SNARE and plasma membrane Qa, Qb and Qc, SNARE proteins. Proteins in the lethal giant larvae protein family, including lethal giant larvae and tomosyn in metazoans and Sro7 in yeast, interact with Q-SNAREs and are emerging as key regulators of polarized, exocytosis. The crystal structure of Sro7 reveals two seven-bladed WD40, beta-propellers followed by a 60-residue-long 'tail', which is bound to, the surface of the amino-terminal propeller. Deletion of the Sro7 tail, enables binding to the Qbc SNARE region of Sec9 and this interaction, inhibits SNARE complex assembly. The N-terminal domain of Sec9 provides a, second, high-affinity Sro7 interaction that is unaffected by the tail. The, results suggest that Sro7 acts as an allosteric regulator of exocytosis, through interactions with factors that control the tail. Sequence, alignments indicate that lethal giant larvae and tomosyn have a, two-beta-propeller fold similar to that of Sro7, but only tomosyn appears, to retain the regulatory tail.
About this Structure
2OAJ is a Single protein structure of sequence from Saccharomyces cerevisiae with and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of the yeast polarity protein Sro7 reveals a SNARE regulatory mechanism., Hattendorf DA, Andreeva A, Gangar A, Brennwald PJ, Weis WI, Nature. 2007 Mar 29;446(7135):567-71. PMID:17392788
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