2oie

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(New page: 200px<br /><applet load="2oie" size="450" color="white" frame="true" align="right" spinBox="true" caption="2oie, resolution 2.2&Aring;" /> '''Crystal structure of ...)
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[[Image:2oie.gif|left|200px]]<br /><applet load="2oie" size="450" color="white" frame="true" align="right" spinBox="true"
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[[Image:2oie.gif|left|200px]]<br /><applet load="2oie" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2oie, resolution 2.2&Aring;" />
caption="2oie, resolution 2.2&Aring;" />
'''Crystal structure of RS21-C6 core segment RSCUT'''<br />
'''Crystal structure of RS21-C6 core segment RSCUT'''<br />
==Overview==
==Overview==
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RS21-C6, which is highly expressed in all vertebrate genomes and green, plants, is proposed to have nucleoside triphosphate pyrophosphohydrolase, activity. Here, we report the crystal structures of the core fragment of, RS21-C6, named RSCUT, and the complex with the substrate 5-methyl dCTP., The refined structure of RSCUT consists mainly of alpha-helices and shows, formation of a tightly associated tetramer. On the basis of the structure, of the RSCUT-m5dCTP complex and the results of pyrophosphatase activity, assays, several key residues involved in the substrate binding of RS21-C6, have been identified. Tetramer formation is shown to be required for, substrate binding.
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RS21-C6, which is highly expressed in all vertebrate genomes and green plants, is proposed to have nucleoside triphosphate pyrophosphohydrolase activity. Here, we report the crystal structures of the core fragment of RS21-C6, named RSCUT, and the complex with the substrate 5-methyl dCTP. The refined structure of RSCUT consists mainly of alpha-helices and shows formation of a tightly associated tetramer. On the basis of the structure of the RSCUT-m5dCTP complex and the results of pyrophosphatase activity assays, several key residues involved in the substrate binding of RS21-C6 have been identified. Tetramer formation is shown to be required for substrate binding.
==About this Structure==
==About this Structure==
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2OIE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2OIE OCA].
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2OIE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OIE OCA].
==Reference==
==Reference==
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Crystal Structure of RS21-C6, Involved in Nucleoside Triphosphate Pyrophosphohydrolysis., Wu B, Liu Y, Zhao Q, Liao S, Zhang J, Bartlam M, Chen W, Rao Z, J Mol Biol. 2007 Jan 26;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17320107 17320107]
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Crystal structure of RS21-C6, involved in nucleoside triphosphate pyrophosphohydrolysis., Wu B, Liu Y, Zhao Q, Liao S, Zhang J, Bartlam M, Chen W, Rao Z, J Mol Biol. 2007 Apr 13;367(5):1405-12. Epub 2007 Jan 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17320107 17320107]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: helix]]
[[Category: helix]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 13:11:58 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:18:51 2008''

Revision as of 16:18, 21 February 2008

Image:2oie.gif

2oie, resolution 2.2Å

Drag the structure with the mouse to rotate

Crystal structure of RS21-C6 core segment RSCUT

Overview

RS21-C6, which is highly expressed in all vertebrate genomes and green plants, is proposed to have nucleoside triphosphate pyrophosphohydrolase activity. Here, we report the crystal structures of the core fragment of RS21-C6, named RSCUT, and the complex with the substrate 5-methyl dCTP. The refined structure of RSCUT consists mainly of alpha-helices and shows formation of a tightly associated tetramer. On the basis of the structure of the RSCUT-m5dCTP complex and the results of pyrophosphatase activity assays, several key residues involved in the substrate binding of RS21-C6 have been identified. Tetramer formation is shown to be required for substrate binding.

About this Structure

2OIE is a Single protein structure of sequence from Mus musculus with as ligand. Full crystallographic information is available from OCA.

Reference

Crystal structure of RS21-C6, involved in nucleoside triphosphate pyrophosphohydrolysis., Wu B, Liu Y, Zhao Q, Liao S, Zhang J, Bartlam M, Chen W, Rao Z, J Mol Biol. 2007 Apr 13;367(5):1405-12. Epub 2007 Jan 26. PMID:17320107

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