2osv
From Proteopedia
(New page: 200px<br /><applet load="2osv" size="450" color="white" frame="true" align="right" spinBox="true" caption="2osv, resolution 1.750Å" /> '''Crystal Structure o...) |
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| - | [[Image:2osv.gif|left|200px]]<br /><applet load="2osv" size=" | + | [[Image:2osv.gif|left|200px]]<br /><applet load="2osv" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2osv, resolution 1.750Å" /> | caption="2osv, resolution 1.750Å" /> | ||
'''Crystal Structure of ZnuA from E. coli'''<br /> | '''Crystal Structure of ZnuA from E. coli'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2OSV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2OSV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OSV OCA]. |
==Reference== | ==Reference== | ||
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[[Category: protein-zinc complex]] | [[Category: protein-zinc complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:15:22 2008'' |
Revision as of 13:15, 23 January 2008
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Crystal Structure of ZnuA from E. coli
Overview
Bacterial ATP-binding cassette transport systems for high-affinity uptake, of zinc and manganese use a cluster 9 solute-binding protein. Structures, of four cluster 9 transport proteins have been determined previously., However, the structural determinants for discrimination between zinc and, manganese remain under discussion. To further investigate the variability, of metal binding sites in bacterial transporters, we have determined the, structure of the zinc-bound transport protein ZnuA from Escherichia coli, to 1.75 A resolution. The overall structure of ZnuA is similar to other, solute-binding transporters. A scaffolding alpha-helix forms the backbone, for two structurally related globular domains. The metal-binding site is, located at the domain interface. The bound zinc ion is coordinated by, three histidine residues (His78, His161 and His225) and one glutamate, residue (Glu77). The functional role of Glu77 for metal binding is, unexpected, because this residue is not conserved in previously determined, structures of zinc and manganese-specific transport proteins. The observed, metal coordination by four protein residues differs significantly from the, zinc-binding site in the ZnuA transporter from Synechocystis 6803, which, binds zinc via three histidine residues. In addition, the E. coli ZnuA, structure reveals the presence of a disulfide bond in the C-terminal, globular domain that is not present in previously determined cluster 9, transport protein structures.
About this Structure
2OSV is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal Structure of the Zinc-binding Transport Protein ZnuA from Escherichia coli Reveals an Unexpected Variation in Metal Coordination., Li H, Jogl G, J Mol Biol. 2007 Mar 15;. PMID:17399739
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