2p7v
From Proteopedia
(New page: 200px<br /><applet load="2p7v" size="450" color="white" frame="true" align="right" spinBox="true" caption="2p7v, resolution 2.600Å" /> '''Crystal structure o...) |
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| - | [[Image:2p7v.jpg|left|200px]]<br /><applet load="2p7v" size=" | + | [[Image:2p7v.jpg|left|200px]]<br /><applet load="2p7v" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2p7v, resolution 2.600Å" /> | caption="2p7v, resolution 2.600Å" /> | ||
'''Crystal structure of the Escherichia coli regulator of sigma 70, Rsd, in complex with sigma 70 domain 4'''<br /> | '''Crystal structure of the Escherichia coli regulator of sigma 70, Rsd, in complex with sigma 70 domain 4'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2P7V is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2P7V is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P7V OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transcription]] | [[Category: transcription]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:23:04 2008'' |
Revision as of 09:23, 23 January 2008
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Crystal structure of the Escherichia coli regulator of sigma 70, Rsd, in complex with sigma 70 domain 4
Overview
The Escherichia coli Rsd protein binds tightly and specifically to the RNA, polymerase (RNAP) sigma(70) factor. Rsd plays a role in alternative sigma, factor-dependent transcription by biasing the competition between, sigma(70) and alternative sigma factors for the available core RNAP. Here, we determined the 2.6 A-resolution X-ray crystal structure of Rsd bound to, sigma(70) domain 4 (sigma(70)(4)), the primary determinant for Rsd binding, within sigma(70). The structure reveals that Rsd binding interferes with, the two primary functions of sigma(70)(4), core RNAP binding and promoter, -35 element binding. Interestingly, the most highly conserved Rsd residues, form a network of interactions through the middle of the Rsd structure, that connect the sigma(70)(4)-binding surface with three cavities exposed, on distant surfaces of Rsd, suggesting functional coupling between, sigma(70)(4) binding and other binding surfaces of Rsd, either for other, proteins or for as yet unknown small molecule effectors. These results, provide a structural basis for understanding the role of Rsd, as well as, its ortholog, AlgQ, a positive regulator of Pseudomonas aeruginosa, virulence, in transcription regulation.
About this Structure
2P7V is a Protein complex structure of sequences from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal Structure of the Escherichia coli Regulator of sigma(70), Rsd, in Complex with sigma(70) Domain 4., Patikoglou GA, Westblade LF, Campbell EA, Lamour V, Lane WJ, Darst SA, J Mol Biol. 2007 Jul 3;. PMID:17681541
Page seeded by OCA on Wed Jan 23 11:23:04 2008
Categories: Escherichia coli | Protein complex | Campbell, E.A. | Darst, S.A. | Lamour, V. | Lane, W.J. | Patikoglou, G.A. | Westblade, L.F. | MG | E. coli rsd-sigma 70 complex | Escherichia coli rsd-sigma 70 complex | Helix-turn-helix | Regulation | Regulator of sigma 70 | Rsd | Rsd-sigma 70 complex | Sigma 70 | Sigma 70 domain 4 | Sigma factor | Transcription
