2p9h
From Proteopedia
(New page: 200px<br /><applet load="2p9h" size="450" color="white" frame="true" align="right" spinBox="true" caption="2p9h, resolution 2.00Å" /> '''High resolution stru...) |
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| - | [[Image:2p9h.jpg|left|200px]]<br /><applet load="2p9h" size=" | + | [[Image:2p9h.jpg|left|200px]]<br /><applet load="2p9h" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2p9h, resolution 2.00Å" /> | caption="2p9h, resolution 2.00Å" /> | ||
'''High resolution structure of the Lactose Repressor bound to IPTG'''<br /> | '''High resolution structure of the Lactose Repressor bound to IPTG'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2P9H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with IPT as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2P9H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=IPT:'>IPT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P9H OCA]. |
==Reference== | ==Reference== | ||
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[[Category: lac repressor]] | [[Category: lac repressor]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:29:10 2008'' |
Revision as of 12:29, 23 January 2008
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High resolution structure of the Lactose Repressor bound to IPTG
Overview
The lac operon is a model system for understanding how effector molecules, regulate transcription and are necessary for allosteric transitions. The, crystal structures of the lac repressor bound to inducer and anti-inducer, molecules provide a model for how these small molecules can modulate, repressor function. The structures of the apo repressor and the repressor, bound to effector molecules are compared in atomic detail. All effectors, examined here bind to the repressor in the same location and are anchored, to the repressor through hydrogen bonds to several hydroxyl groups of the, sugar ring. Inducer molecules form a more extensive hydrogen-bonding, network compared to anti-inducers and neutral effector molecules. The, structures of these effector molecules suggest that the O6 hydroxyl on the, galactoside is essential for establishing a water-mediated hydrogen, bonding network that bridges the N-terminal and C-terminal sub-domains., The altered hydrogen bonding can account in part for the different, structural conformations of the repressor, and is vital for the allosteric, transition.
About this Structure
2P9H is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
Structural analysis of lac repressor bound to allosteric effectors., Daber R, Stayrook S, Rosenberg A, Lewis M, J Mol Biol. 2007 Jul 20;370(4):609-19. Epub 2007 Apr 19. PMID:17543986
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