2wdh
From Proteopedia
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===STRUCTURE OF THE THERMUS THERMOPHILUS 70S RIBOSOME IN COMPLEX WITH MRNA, PAROMOMYCIN, ACYLATED A-SITE TRNA, DEACYLATED P-SITE TRNA, AND E-SITE TRNA. THIS FILE CONTAINS THE 30S SUBUNIT A-,P-, AND E-SITE TRNAS AND PAROMOMYCIN FOR MOLECULE II.=== | ===STRUCTURE OF THE THERMUS THERMOPHILUS 70S RIBOSOME IN COMPLEX WITH MRNA, PAROMOMYCIN, ACYLATED A-SITE TRNA, DEACYLATED P-SITE TRNA, AND E-SITE TRNA. THIS FILE CONTAINS THE 30S SUBUNIT A-,P-, AND E-SITE TRNAS AND PAROMOMYCIN FOR MOLECULE II.=== | ||
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==About this Structure== | ==About this Structure== | ||
2WDH is a 25 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WDH OCA]. | 2WDH is a 25 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WDH OCA]. | ||
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| + | ==Reference== | ||
| + | <ref group="xtra">PMID:19363482</ref><references group="xtra"/> | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
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[[Category: Zinc-finger]] | [[Category: Zinc-finger]] | ||
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Revision as of 17:35, 29 April 2009
STRUCTURE OF THE THERMUS THERMOPHILUS 70S RIBOSOME IN COMPLEX WITH MRNA, PAROMOMYCIN, ACYLATED A-SITE TRNA, DEACYLATED P-SITE TRNA, AND E-SITE TRNA. THIS FILE CONTAINS THE 30S SUBUNIT A-,P-, AND E-SITE TRNAS AND PAROMOMYCIN FOR MOLECULE II.
Protein synthesis is catalyzed in the peptidyl transferase center (PTC), located in the large (50S) subunit of the ribosome. No high-resolution structure of the intact ribosome has contained a complete active site including both A- and P-site tRNAs. In addition, although past structures of the 50S subunit have found no ordered proteins at the PTC, biochemical evidence suggests that specific proteins are capable of interacting with the 3' ends of tRNA ligands. Here we present structures, at 3.6-A and 3.5-A resolution respectively, of the 70S ribosome in complex with A- and P-site tRNAs that mimic pre- and post-peptidyl-transfer states. These structures demonstrate that the PTC is very similar between the 50S subunit and the intact ribosome. They also reveal interactions between the ribosomal proteins L16 and L27 and the tRNA substrates, helping to elucidate the role of these proteins in peptidyl transfer.
Insights into substrate stabilization from snapshots of the peptidyl transferase center of the intact 70S ribosome., Voorhees RM, Weixlbaumer A, Loakes D, Kelley AC, Ramakrishnan V, Nat Struct Mol Biol. 2009 May;16(5):528-33. Epub 2009 Apr 12. PMID:19363482
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2WDH is a 25 chains structure of sequences from Escherichia coli and Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
- Voorhees RM, Weixlbaumer A, Loakes D, Kelley AC, Ramakrishnan V. Insights into substrate stabilization from snapshots of the peptidyl transferase center of the intact 70S ribosome. Nat Struct Mol Biol. 2009 May;16(5):528-33. Epub 2009 Apr 12. PMID:19363482 doi:10.1038/nsmb.1577
Page seeded by OCA on Wed Apr 29 20:35:48 2009
Categories: Escherichia coli | Thermus thermophilus | Kelley, A C. | Loakes, D. | Ramakrishnan, V. | Voorhees, R M. | Weixlbaumer, A. | Coiled coil | Metal-binding | Mrna | Ribonucleoprotein | Ribosomal protein | Ribosome | Rna-binding | Rrna-binding | Translation | Trna | Trna-binding | Zinc | Zinc-finger
