2px4
From Proteopedia
(New page: 200px<br /><applet load="2px4" size="450" color="white" frame="true" align="right" spinBox="true" caption="2px4, resolution 2.20Å" /> '''Crystal structure of...) |
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| - | [[Image:2px4.jpg|left|200px]]<br /><applet load="2px4" size=" | + | [[Image:2px4.jpg|left|200px]]<br /><applet load="2px4" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2px4, resolution 2.20Å" /> | caption="2px4, resolution 2.20Å" /> | ||
'''Crystal structure of the Murray Valley Encephalitis Virus NS5 2'-O Methyltransferase domain in complex with SAH (Monoclinic form 2)'''<br /> | '''Crystal structure of the Murray Valley Encephalitis Virus NS5 2'-O Methyltransferase domain in complex with SAH (Monoclinic form 2)'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2PX4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Murray_valley_encephalitis_virus Murray valley encephalitis virus] with CL, SO4, UNX, SAH and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] Full crystallographic information is available from [http:// | + | 2PX4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Murray_valley_encephalitis_virus Murray valley encephalitis virus] with <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=UNX:'>UNX</scene>, <scene name='pdbligand=SAH:'>SAH</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PX4 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:23:15 2008'' |
Revision as of 12:23, 23 January 2008
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Crystal structure of the Murray Valley Encephalitis Virus NS5 2'-O Methyltransferase domain in complex with SAH (Monoclinic form 2)
Overview
We have determined the high resolution crystal structure of the, methyltransferase domain of the NS5 polypeptide from the Murray Valley, encephalitis virus. This domain is unusual in having both the N7 and 2'-O, methyltransferase activity required for Cap 1 synthesis. We have also, determined structures for complexes of this domain with nucleotides and, cap analogues providing information on cap binding, based on which we, suggest a model of how the sequential methylation of the N7 and 2'-O, groups of the cap may be coordinated.
About this Structure
2PX4 is a Single protein structure of sequence from Murray valley encephalitis virus with , , , and as ligands. Active as RNA-directed RNA polymerase, with EC number 2.7.7.48 Full crystallographic information is available from OCA.
Reference
Crystal structure of the Murray Valley encephalitis virus NS5 methyltransferase domain in complex with cap analogues., Assenberg R, Ren J, Verma A, Walter TS, Alderton D, Hurrelbrink RJ, Fuller SD, Bressanelli S, Owens RJ, Stuart DI, Grimes JM, J Gen Virol. 2007 Aug;88(Pt 8):2228-36. PMID:17622627
Page seeded by OCA on Wed Jan 23 14:23:15 2008
Categories: Murray valley encephalitis virus | RNA-directed RNA polymerase | Single protein | Alderton, D. | Assenberg, R. | Fuller, S.D. | Grimes, J.M. | Hurrelbrink, R.J. | OPPF, Oxford.Protein.Production.Facility. | Owens, R.J. | Ren, J. | Stuart, D.I. | Verma, A. | Walter, T.S. | CL | GOL | SAH | SO4 | UNX | Methyltransferase | Oppf | Oxford protein production facility | Sah | Structural genomics
