This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
User:Susana Retamal/Sandbox1
From Proteopedia
(Difference between revisions)
| Line 3: | Line 3: | ||
=Background Information= | =Background Information= | ||
| - | Rsp1275 is a member of the Fnr-Crp family of transcriptional regulators. Homologues of the Fnr protein from ''E. coli'' have been identified in a variety of taxonomically <1> diverse bacterial species. The bacterium ''Rhodobacter spheroides 2.4.1'' encodes 8 members of this family, however only 2 have known function. The modeling of 7 of them were successfully obtained using 3D-Jigsaw, all being very similar in structure to Crp (the only one with crystal structure solved). The DNA binding domain helix-turn-helix is located in the C-terminal, and the N-terminal part contains the allosteric effector domain. Rsp1275 has been linked with salt-tolerance in ''Rhodobacter spheroides''. Different genes coding for the Fnr-Crp proteins of unknown function have been cloned and a multicopy test was performed to evaluate these as potential regulators of NaCl-responsive genes. | + | Rsp1275 is a member of the Fnr-Crp family of transcriptional regulators. Homologues of the Fnr protein from ''E. coli'' have been identified in a variety of taxonomically <ref>1) Spiro, S. 1994. <u>The FNR family of transcriptional regulators.</u>. Antonie van Leeuwenhoek 66:23–36. <u> diverse bacterial species. The bacterium ''Rhodobacter spheroides 2.4.1'' encodes 8 members of this family, however only 2 have known function. The modeling of 7 of them were successfully obtained using 3D-Jigsaw, all being very similar in structure to Crp (the only one with crystal structure solved). The DNA binding domain helix-turn-helix is located in the C-terminal, and the N-terminal part contains the allosteric effector domain. Rsp1275 has been linked with salt-tolerance in ''Rhodobacter spheroides''. Different genes coding for the Fnr-Crp proteins of unknown function have been cloned and a multicopy test was performed to evaluate these as potential regulators of NaCl-responsive genes. |
''Structure of the Proposed rsp1275'''<applet load='rsp1275.pdb' size='300' color='black' frame='true' align='right' caption='3D Image of proposed rsp1275 protein'/> | ''Structure of the Proposed rsp1275'''<applet load='rsp1275.pdb' size='300' color='black' frame='true' align='right' caption='3D Image of proposed rsp1275 protein'/> | ||
| Line 27: | Line 27: | ||
Theoretical pI: 6.10 | Theoretical pI: 6.10 | ||
| - | Globe Prediction: it appears as compact, as a globular doimain. | + | Globe Prediction: it appears as compact, as a globular doimain. <ref>2)PredictProtein: B Rost, G Yachdav and J Liu (2004) <u> |
Secondary Structure: | Secondary Structure: | ||
| Line 306: | Line 306: | ||
| - | =References= | + | ==References== |
| - | + | 1) Spiro, S. 1994. <u>The FNR family of transcriptional regulators.</u>. Antonie van Leeuwenhoek 66:23–36. | |
| - | + | 2) PredictProtein: B Rost, G Yachdav and J Liu (2004) <u> The PredictProtein Server.</u> Nucleic Acids Research 32(Web Server issue):W321-W326. | |
Revision as of 14:24, 27 April 2009
- User:Susana Retamal/Sandbox1
Background Information
Rsp1275 is a member of the Fnr-Crp family of transcriptional regulators. Homologues of the Fnr protein from E. coli have been identified in a variety of taxonomically [1]
