2qca
From Proteopedia
(New page: 200px<br /><applet load="2qca" size="450" color="white" frame="true" align="right" spinBox="true" caption="2qca, resolution 1.33Å" /> '''A New Crystal Form o...) |
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| - | [[Image:2qca.jpg|left|200px]]<br /><applet load="2qca" size=" | + | [[Image:2qca.jpg|left|200px]]<br /><applet load="2qca" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2qca, resolution 1.33Å" /> | caption="2qca, resolution 1.33Å" /> | ||
'''A New Crystal Form of Bovine Pancreatic RNase A in Complex with 2'-Deoxyguanosine-5'-monophosphate'''<br /> | '''A New Crystal Form of Bovine Pancreatic RNase A in Complex with 2'-Deoxyguanosine-5'-monophosphate'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2QCA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with DGP as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http:// | + | 2QCA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=DGP:'>DGP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QCA OCA]. |
==Reference== | ==Reference== | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:37:37 2008'' |
Revision as of 11:37, 23 January 2008
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A New Crystal Form of Bovine Pancreatic RNase A in Complex with 2'-Deoxyguanosine-5'-monophosphate
Overview
The structure of bovine pancreatic RNase A has been determined in complex, with 2'-deoxyguanosine-5'-monophosphate (dGMP) at 1.33 A resolution at, room temperature in a previously unreported unit cell belonging to space, group P3(1). There are two molecules of nucleotide per enzyme molecule, one of which lies in the active-site cleft in the productive binding mode, whilst the guanine base of the other dGMP occupies the pyrimidine-specific, binding site in a nonproductive mode such that it forms hydrogen bonds to, the phosphate group of the first dGMP. This is the first RNase A structure, containing a guanine base in the B2 binding site. Each dGMP molecule is, involved in intermolecular interactions with adjacent RNase A molecules in, the lattice and the two nucleotides appear to direct the formation of the, crystal lattice. Because GMP may be produced during degradation of RNA, this association could represent an inhibitor complex and thereby affect, the observed enzyme kinetics.
About this Structure
2QCA is a Single protein structure of sequence from Bos taurus with as ligand. Active as Pancreatic ribonuclease, with EC number 3.1.27.5 Full crystallographic information is available from OCA.
Reference
A new crystal form of bovine pancreatic RNase A in complex with 2'-deoxyguanosine-5'-monophosphate., Larson SB, Day JS, Cudney R, McPherson A, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Sep 1;63(Pt, 9):728-33. Epub 2007 Aug 31. PMID:17768339
Page seeded by OCA on Wed Jan 23 13:37:37 2008
Categories: Bos taurus | Pancreatic ribonuclease | Single protein | CHTSB, Center.for.High-Throughput.Structural.Biology. | Cudney, R. | Day, J.S. | Larson, S.B. | McPherson, A. | DGP | Center for high-throughput structural biology | Chtsb | Hydrolase | Protein structure initiative | Protein-nucleotide complex | Psi-2 | Ribonuclease | Structural genomics
