2qi9
From Proteopedia
(New page: 200px<br /><applet load="2qi9" size="450" color="white" frame="true" align="right" spinBox="true" caption="2qi9, resolution 2.600Å" /> '''ABC-transporter Btu...) |
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| - | [[Image:2qi9.jpg|left|200px]]<br /><applet load="2qi9" size=" | + | [[Image:2qi9.jpg|left|200px]]<br /><applet load="2qi9" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2qi9, resolution 2.600Å" /> | caption="2qi9, resolution 2.600Å" /> | ||
'''ABC-transporter BtuCD in complex with its periplasmic binding protein BtuF'''<br /> | '''ABC-transporter BtuCD in complex with its periplasmic binding protein BtuF'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2QI9 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with PO4, SO4, 1PE and PEG as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Vitamin_B12-transporting_ATPase Vitamin B12-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.33 3.6.3.33] Full crystallographic information is available from [http:// | + | 2QI9 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=PO4:'>PO4</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=1PE:'>1PE</scene> and <scene name='pdbligand=PEG:'>PEG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Vitamin_B12-transporting_ATPase Vitamin B12-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.33 3.6.3.33] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QI9 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transport]] | [[Category: transport]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:34:48 2008'' |
Revision as of 10:34, 23 January 2008
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ABC-transporter BtuCD in complex with its periplasmic binding protein BtuF
Overview
BtuCD is an adenosine triphosphate-binding cassette (ABC) transporter that, translocates vitamin B12 from the periplasmic binding protein BtuF into, the cytoplasm of Escherichia coli. The 2.6 angstrom crystal structure of a, complex BtuCD-F reveals substantial conformational changes as compared, with the previously reported structures of BtuCD and BtuF. The lobes of, BtuF are spread apart, and B12 is displaced from the binding pocket. The, transmembrane BtuC subunits reveal two distinct conformations, and the, translocation pathway is closed to both sides of the membrane. Electron, paramagnetic resonance spectra of spin-labeled cysteine mutants, reconstituted in proteoliposomes are consistent with the conformation of, BtuCD-F that was observed in the crystal structure. A comparison with, BtuCD and the homologous HI1470/71 protein suggests that the structure of, BtuCD-F may reflect a posttranslocation intermediate.
About this Structure
2QI9 is a Protein complex structure of sequences from Escherichia coli with , , and as ligands. Active as Vitamin B12-transporting ATPase, with EC number 3.6.3.33 Full crystallographic information is available from OCA.
Reference
Asymmetry in the structure of the ABC transporter-binding protein complex BtuCD-BtuF., Hvorup RN, Goetz BA, Niederer M, Hollenstein K, Perozo E, Locher KP, Science. 2007 Sep 7;317(5843):1387-90. Epub 2007 Aug 2. PMID:17673622
Page seeded by OCA on Wed Jan 23 12:34:48 2008
Categories: Escherichia coli | Protein complex | Vitamin B12-transporting ATPase | Goetz, B.A. | Hollenstein, K. | Hvorup, R.N. | Locher, K.P. | Niederer, M. | Perozo, E. | 1PE | PEG | PO4 | SO4 | Atp-binding | Hydrolase | Inner membrane | Membrane | Membrane protein | Nucleotide-binding | Periplasm | Transmembrane | Transport
