2v8n
From Proteopedia
(New page: 200px<br /><applet load="2v8n" size="450" color="white" frame="true" align="right" spinBox="true" caption="2v8n, resolution 3.60Å" /> '''WILD-TYPE STRUCTURE ...) |
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| - | [[Image:2v8n.jpg|left|200px]]<br /><applet load="2v8n" size=" | + | [[Image:2v8n.jpg|left|200px]]<br /><applet load="2v8n" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2v8n, resolution 3.60Å" /> | caption="2v8n, resolution 3.60Å" /> | ||
'''WILD-TYPE STRUCTURE OF LACTOSE PERMEASE'''<br /> | '''WILD-TYPE STRUCTURE OF LACTOSE PERMEASE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2V8N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 2V8N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V8N OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transport protein]] | [[Category: transport protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:58:51 2008'' |
Revision as of 10:58, 23 January 2008
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WILD-TYPE STRUCTURE OF LACTOSE PERMEASE
Overview
Here we describe an x-ray structure of wild-type lactose permease (LacY), from Escherichia coli determined by manipulating phospholipid content, during crystallization. The structure exhibits the same global fold as the, previous x-ray structures of a mutant that binds sugar but cannot catalyze, translocation across the membrane. LacY is organized into two six-helix, bundles with twofold pseudosymmetry separated by a large interior, hydrophilic cavity open only to the cytoplasmic side and containing the, side chains important for sugar and H(+) binding. To initiate transport, binding of sugar and/or an H(+) electrochemical gradient increases the, probability of opening on the periplasmic side. Because the inward-facing, conformation represents the lowest free-energy state, the rate-limiting, step for transport may be the conformational change leading to the, outward-facing conformation.
About this Structure
2V8N is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structural determination of wild-type lactose permease., Guan L, Mirza O, Verner G, Iwata S, Kaback HR, Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15294-8. Epub 2007 Sep 19. PMID:17881559
Page seeded by OCA on Wed Jan 23 12:58:51 2008
