2v74

From Proteopedia

Revision as of 15:48, 30 October 2007

CRYSTAL STRUCTURE OF COACTIVATOR-ASSOCIATED ARGININE METHYLTRANSFERASE 1 (CARM1), IN COMPLEX WITH S-ADENOSYL-HOMOCYSTEINE

Overview

Coactivator-associated arginine methyltransferase (CARM1) is a, transcriptional coactivator that methylates Arg17 and Arg26 in histone H3., CARM1 contains a conserved protein arginine methyltransferase (PRMT), catalytic core flanked by unique pre- and post-core regions. The crystal, structures of the CARM1 catalytic core in the apo and holo states reveal, cofactor-dependent formation of a substrate-binding groove providing a, specific access channel for arginine to the active site. The groove is, supported by the first eight residues of the post-core region, (C-extension), not present in other PRMTs. In vitro methylation assays, show that the C-extension is essential for all histone H3 methylation, activity, whereas the pre-core region is required for methylation of, Arg26, but not Arg17. ... [(full description)]

About this Structure

2V74 is a [Single protein] structure of sequence from [Mus musculus] with SAH as [ligand]. Active as [Histone-arginine N-methyltransferase], with EC number [2.1.1.125]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Insights into histone code syntax from structural and biochemical studies of CARM1 methyltransferase., Yue WW, Hassler M, Roe SM, Thompson-Vale V, Pearl LH, EMBO J. 2007 Sep 20;. PMID:17882261

Page seeded by OCA on Tue Oct 30 17:53:09 2007