1ick
From Proteopedia
(New page: 200px<br /><applet load="1ick" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ick, resolution 0.95Å" /> '''LEFT-HANDED Z-DNA HE...) |
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| - | [[Image:1ick.jpg|left|200px]]<br /><applet load="1ick" size=" | + | [[Image:1ick.jpg|left|200px]]<br /><applet load="1ick" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ick, resolution 0.95Å" /> | caption="1ick, resolution 0.95Å" /> | ||
'''LEFT-HANDED Z-DNA HEXAMER DUPLEX D(CGCGCG)2'''<br /> | '''LEFT-HANDED Z-DNA HEXAMER DUPLEX D(CGCGCG)2'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of a left-handed Z-DNA hexamer duplex d(CGCGCG)(2) | + | The crystal structure of a left-handed Z-DNA hexamer duplex d(CGCGCG)(2) has been solved based on the anomalous diffraction signal of inherent P atoms using data collected at the single wavelength of 1.54 A. The anomalous signal of about 2% of the total diffracted intensity, constant for all nucleic acids, may be generally useful for solving crystal structures of DNA and RNA oligomers. The multiplicity of intensity measurements is shown to crucially affect the data quality and the ability to solve the phase problem. The anisotropic model refined to an R factor of 8.9% at 0.95 A resolution. |
==About this Structure== | ==About this Structure== | ||
| - | 1ICK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with SPM and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1ICK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=SPM:'>SPM</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ICK OCA]. |
==Reference== | ==Reference== | ||
Anomalous signal of phosphorus used for phasing DNA oligomer: importance of data redundancy., Dauter Z, Adamiak DA, Acta Crystallogr D Biol Crystallogr. 2001 Jul;57(Pt 7):990-5. Epub 2001, Jun 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11418767 11418767] | Anomalous signal of phosphorus used for phasing DNA oligomer: importance of data redundancy., Dauter Z, Adamiak DA, Acta Crystallogr D Biol Crystallogr. 2001 Jul;57(Pt 7):990-5. Epub 2001, Jun 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11418767 11418767] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Adamiak, D | + | [[Category: Adamiak, D A.]] |
[[Category: Dauter, Z.]] | [[Category: Dauter, Z.]] | ||
[[Category: MG]] | [[Category: MG]] | ||
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[[Category: left-handed z-dna oligomer]] | [[Category: left-handed z-dna oligomer]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:10:31 2008'' |
Revision as of 11:10, 21 February 2008
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LEFT-HANDED Z-DNA HEXAMER DUPLEX D(CGCGCG)2
Overview
The crystal structure of a left-handed Z-DNA hexamer duplex d(CGCGCG)(2) has been solved based on the anomalous diffraction signal of inherent P atoms using data collected at the single wavelength of 1.54 A. The anomalous signal of about 2% of the total diffracted intensity, constant for all nucleic acids, may be generally useful for solving crystal structures of DNA and RNA oligomers. The multiplicity of intensity measurements is shown to crucially affect the data quality and the ability to solve the phase problem. The anisotropic model refined to an R factor of 8.9% at 0.95 A resolution.
About this Structure
1ICK is a Protein complex structure of sequences from [1] with and as ligands. Full crystallographic information is available from OCA.
Reference
Anomalous signal of phosphorus used for phasing DNA oligomer: importance of data redundancy., Dauter Z, Adamiak DA, Acta Crystallogr D Biol Crystallogr. 2001 Jul;57(Pt 7):990-5. Epub 2001, Jun 21. PMID:11418767
Page seeded by OCA on Thu Feb 21 13:10:31 2008
