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1r3o
From Proteopedia
(New page: 200px<br /><applet load="1r3o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r3o, resolution 1.90Å" /> '''Crystal structure of...) |
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| - | [[Image:1r3o.gif|left|200px]]<br /><applet load="1r3o" size=" | + | [[Image:1r3o.gif|left|200px]]<br /><applet load="1r3o" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1r3o, resolution 1.90Å" /> | caption="1r3o, resolution 1.90Å" /> | ||
'''Crystal structure of the first RNA duplex in L-conformation at 1.9A resolution'''<br /> | '''Crystal structure of the first RNA duplex in L-conformation at 1.9A resolution'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Nucleic acid molecules in the mirror image or L-configuration are unknown | + | Nucleic acid molecules in the mirror image or L-configuration are unknown in nature and are extraordinarily resistant to biological degradation. The identification of functional L-oligonucleotides called Spiegelmers offers a novel approach for drug discovery based on RNA. The sequence r(CUGGGCGG).r(CCGCCUGG) was chosen as a model system for structural analysis of helices in the L-configuration as the structure of the D-form of this sequence has previously been determined in structural studies of 5S RNA domains, in particular domain E of the Thermus flavus 5S rRNA [Perbandt et al. (2001), Acta Cryst. D57, 219-224]. Unexpectedly, the results of crystallization trials showed little similarity between the D- and the L-forms of the duplex in either the crystallization hits or the diffraction performance. The crystal structure of this L-RNA duplex has been determined at 1.9 A resolution with R(work) and R(free) of 23.8 and 28.6%, respectively. The crystals belong to space group R32, with unit-cell parameters a = 45.7, c = 264.6 A. Although there are two molecules in the asymmetric unit rather than one, the structure of the L-form arranges helical pairs in a head-to-tail fashion to form pseudo-continuous infinite helices in the crystal as in the D-form. On the other hand, the wobble-like G.C(+) base pair seen in the D-RNA analogue does not appear in the L-RNA duplex, which forms a regular double-helical structure with typical Watson-Crick base pairing. |
==About this Structure== | ==About this Structure== | ||
| - | 1R3O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1R3O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R3O OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Betzel, C.]] | [[Category: Betzel, C.]] | ||
| - | [[Category: Erdmann, V | + | [[Category: Erdmann, V A.]] |
[[Category: Klussmann, S.]] | [[Category: Klussmann, S.]] | ||
[[Category: Perbandt, M.]] | [[Category: Perbandt, M.]] | ||
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[[Category: l-rna]] | [[Category: l-rna]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:46:48 2008'' |
Revision as of 12:46, 21 February 2008
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Crystal structure of the first RNA duplex in L-conformation at 1.9A resolution
Overview
Nucleic acid molecules in the mirror image or L-configuration are unknown in nature and are extraordinarily resistant to biological degradation. The identification of functional L-oligonucleotides called Spiegelmers offers a novel approach for drug discovery based on RNA. The sequence r(CUGGGCGG).r(CCGCCUGG) was chosen as a model system for structural analysis of helices in the L-configuration as the structure of the D-form of this sequence has previously been determined in structural studies of 5S RNA domains, in particular domain E of the Thermus flavus 5S rRNA [Perbandt et al. (2001), Acta Cryst. D57, 219-224]. Unexpectedly, the results of crystallization trials showed little similarity between the D- and the L-forms of the duplex in either the crystallization hits or the diffraction performance. The crystal structure of this L-RNA duplex has been determined at 1.9 A resolution with R(work) and R(free) of 23.8 and 28.6%, respectively. The crystals belong to space group R32, with unit-cell parameters a = 45.7, c = 264.6 A. Although there are two molecules in the asymmetric unit rather than one, the structure of the L-form arranges helical pairs in a head-to-tail fashion to form pseudo-continuous infinite helices in the crystal as in the D-form. On the other hand, the wobble-like G.C(+) base pair seen in the D-RNA analogue does not appear in the L-RNA duplex, which forms a regular double-helical structure with typical Watson-Crick base pairing.
About this Structure
1R3O is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
First look at RNA in L-configuration., Vallazza M, Perbandt M, Klussmann S, Rypniewski W, Einspahr HM, Erdmann VA, Betzel Ch, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):1-7. Epub 2003 Dec, 18. PMID:14684885
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