177d

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'''SOLUTION STRUCTURE AND HYDRATION PATTERNS OF A PYRIMIDINE(DOT)PURINE(DOT)PYRIMIDINE DNA TRIPLEX CONTAINING A NOVEL T(DOT)CG TRIPLE'''<br />
'''SOLUTION STRUCTURE AND HYDRATION PATTERNS OF A PYRIMIDINE(DOT)PURINE(DOT)PYRIMIDINE DNA TRIPLEX CONTAINING A NOVEL T(DOT)CG TRIPLE'''<br />
==Overview==
==Overview==
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The solution structure of a pyrimidine.purine.pyrimidine DNA triplex, containing a novel T.CG base-triple has been determined via two and, three-dimensional NMR spectroscopy and restrained molecular dynamics, simulations incorporating explicit solvent and counter-ions. The T.CG, triple, which expands the triplex code, is stabilized by a single hydrogen, bond between the O-2 atom of thymine and the free amino proton of cytosine, in the Watson-Crick C.G base-pair. This hydrogen bonding alignment, produces large variations in helical twist at the dinucleotide steps, involving the thymine residue. Localized structural perturbations in the, purine-rich strand of the molecule are observed around the cytosine, residue in the T.CG triple. Globally, the triplex resembles the solution, structure of a previously solved pyrimidine.purine.pyrimidine DNA triplex, containing an unusual G.TA triple. Also conserved are the sites and, patterns of hydration in the two triplexes.
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The solution structure of a pyrimidine.purine.pyrimidine DNA triplex containing a novel T.CG base-triple has been determined via two and three-dimensional NMR spectroscopy and restrained molecular dynamics simulations incorporating explicit solvent and counter-ions. The T.CG triple, which expands the triplex code, is stabilized by a single hydrogen bond between the O-2 atom of thymine and the free amino proton of cytosine in the Watson-Crick C.G base-pair. This hydrogen bonding alignment produces large variations in helical twist at the dinucleotide steps involving the thymine residue. Localized structural perturbations in the purine-rich strand of the molecule are observed around the cytosine residue in the T.CG triple. Globally, the triplex resembles the solution structure of a previously solved pyrimidine.purine.pyrimidine DNA triplex containing an unusual G.TA triple. Also conserved are the sites and patterns of hydration in the two triplexes.
==About this Structure==
==About this Structure==
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177D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=177D OCA].
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177D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=177D OCA].
==Reference==
==Reference==
Solution structure and hydration patterns of a pyrimidine.purine.pyrimidine DNA triplex containing a novel T.CG base-triple., Radhakrishnan I, Patel DJ, J Mol Biol. 1994 Aug 26;241(4):600-19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8057381 8057381]
Solution structure and hydration patterns of a pyrimidine.purine.pyrimidine DNA triplex containing a novel T.CG base-triple., Radhakrishnan I, Patel DJ, J Mol Biol. 1994 Aug 26;241(4):600-19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8057381 8057381]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Patel, D.J.]]
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[[Category: Patel, D J.]]
[[Category: Radhakrishnan, I.]]
[[Category: Radhakrishnan, I.]]
[[Category: dna]]
[[Category: dna]]
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[[Category: triplex]]
[[Category: triplex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:06:09 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:38:56 2008''

Revision as of 09:39, 21 February 2008

Image:177d.jpg

177d

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SOLUTION STRUCTURE AND HYDRATION PATTERNS OF A PYRIMIDINE(DOT)PURINE(DOT)PYRIMIDINE DNA TRIPLEX CONTAINING A NOVEL T(DOT)CG TRIPLE

Overview

The solution structure of a pyrimidine.purine.pyrimidine DNA triplex containing a novel T.CG base-triple has been determined via two and three-dimensional NMR spectroscopy and restrained molecular dynamics simulations incorporating explicit solvent and counter-ions. The T.CG triple, which expands the triplex code, is stabilized by a single hydrogen bond between the O-2 atom of thymine and the free amino proton of cytosine in the Watson-Crick C.G base-pair. This hydrogen bonding alignment produces large variations in helical twist at the dinucleotide steps involving the thymine residue. Localized structural perturbations in the purine-rich strand of the molecule are observed around the cytosine residue in the T.CG triple. Globally, the triplex resembles the solution structure of a previously solved pyrimidine.purine.pyrimidine DNA triplex containing an unusual G.TA triple. Also conserved are the sites and patterns of hydration in the two triplexes.

About this Structure

177D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Solution structure and hydration patterns of a pyrimidine.purine.pyrimidine DNA triplex containing a novel T.CG base-triple., Radhakrishnan I, Patel DJ, J Mol Biol. 1994 Aug 26;241(4):600-19. PMID:8057381

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