1ehv
From Proteopedia
(New page: 200px<br /><applet load="1ehv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ehv, resolution 1.8Å" /> '''A NEW CRYSTAL STRUCTU...) |
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| - | [[Image:1ehv.gif|left|200px]]<br /><applet load="1ehv" size=" | + | [[Image:1ehv.gif|left|200px]]<br /><applet load="1ehv" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ehv, resolution 1.8Å" /> | caption="1ehv, resolution 1.8Å" /> | ||
'''A NEW CRYSTAL STRUCTURE FOR THE DODECAMER C-G-C-G-A-A-T-T-C-G-C-G: SYMMETRY EFFECTS ON SEQUENCE-DEPENDENT DNA STRUCTURE'''<br /> | '''A NEW CRYSTAL STRUCTURE FOR THE DODECAMER C-G-C-G-A-A-T-T-C-G-C-G: SYMMETRY EFFECTS ON SEQUENCE-DEPENDENT DNA STRUCTURE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The dodecanucleotide d(CGCGAATTCGCG) has been crystallised in the space | + | The dodecanucleotide d(CGCGAATTCGCG) has been crystallised in the space group P3(2)12, representing a new crystal form for this sequence. The structure has been solved by molecular replacement and refined at 1.8 A resolution. The present structure contrasts with previous ones for this sequence since it is situated on a crystallographic 2-fold axis, and the crystal symmetry reflects the palindromic nature of this sequence. Some features accord with previous observations, notably that the minor groove is hydrated with a continuous spine of solvent. There is no evidence of alkali metal ions within this spine. The minor groove retains its narrow width, although it is now symmetric and extends over the A/T tract. Various base and base-pair morphological parameters have been examined. Their values do not show significant correlations with earlier reports, suggesting that crystal packing effects on them are more dominant than has been hitherto realised. |
==About this Structure== | ==About this Structure== | ||
| - | 1EHV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1EHV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EHV OCA]. |
==Reference== | ==Reference== | ||
A new crystal form for the dodecamer C-G-C-G-A-A-T-T-C-G-C-G: symmetry effects on sequence-dependent DNA structure., Johansson E, Parkinson G, Neidle S, J Mol Biol. 2000 Jul 14;300(3):551-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10884351 10884351] | A new crystal form for the dodecamer C-G-C-G-A-A-T-T-C-G-C-G: symmetry effects on sequence-dependent DNA structure., Johansson E, Parkinson G, Neidle S, J Mol Biol. 2000 Jul 14;300(3):551-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10884351 10884351] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Johansson, E | + | [[Category: Johansson, E M.]] |
[[Category: crystal packing]] | [[Category: crystal packing]] | ||
[[Category: dickerson dodecamer]] | [[Category: dickerson dodecamer]] | ||
[[Category: dna]] | [[Category: dna]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:27:53 2008'' |
Revision as of 10:27, 21 February 2008
|
A NEW CRYSTAL STRUCTURE FOR THE DODECAMER C-G-C-G-A-A-T-T-C-G-C-G: SYMMETRY EFFECTS ON SEQUENCE-DEPENDENT DNA STRUCTURE
Overview
The dodecanucleotide d(CGCGAATTCGCG) has been crystallised in the space group P3(2)12, representing a new crystal form for this sequence. The structure has been solved by molecular replacement and refined at 1.8 A resolution. The present structure contrasts with previous ones for this sequence since it is situated on a crystallographic 2-fold axis, and the crystal symmetry reflects the palindromic nature of this sequence. Some features accord with previous observations, notably that the minor groove is hydrated with a continuous spine of solvent. There is no evidence of alkali metal ions within this spine. The minor groove retains its narrow width, although it is now symmetric and extends over the A/T tract. Various base and base-pair morphological parameters have been examined. Their values do not show significant correlations with earlier reports, suggesting that crystal packing effects on them are more dominant than has been hitherto realised.
About this Structure
1EHV is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
A new crystal form for the dodecamer C-G-C-G-A-A-T-T-C-G-C-G: symmetry effects on sequence-dependent DNA structure., Johansson E, Parkinson G, Neidle S, J Mol Biol. 2000 Jul 14;300(3):551-61. PMID:10884351
Page seeded by OCA on Thu Feb 21 12:27:53 2008
