1a9h
From Proteopedia
(New page: 200px<br /><applet load="1a9h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a9h" /> '''APURINIC DNA WITH BOUND WATER AT THE DAMAGED...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1a9h.jpg|left|200px]]<br /><applet load="1a9h" size=" | + | [[Image:1a9h.jpg|left|200px]]<br /><applet load="1a9h" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1a9h" /> | caption="1a9h" /> | ||
'''APURINIC DNA WITH BOUND WATER AT THE DAMAGED SITE AND O2 OF CYTOSINE, BETA FORM, NMR, 1 STRUCTURE'''<br /> | '''APURINIC DNA WITH BOUND WATER AT THE DAMAGED SITE AND O2 OF CYTOSINE, BETA FORM, NMR, 1 STRUCTURE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Natural and exogenous processes can give rise to abasic sites with either | + | Natural and exogenous processes can give rise to abasic sites with either a purine or pyrimidine as the base on the opposing strand. The solution state structures of the apyrimidinic DNA duplex, with D6 indicating an abasic site, [sequence: see text] referred to as AD, and the apurinic DNA duplex with a dC17, referred to as CD, have been determined. A particularly striking difference is that the abasic site in CD is predominantly a beta hemiacetal, whereas in AD the alpha and beta forms are equally present. Hydrogen bonding with water by the abasic site and the base on the opposite strand appears to play a large role in determining the structure near the damaged site. Comparison of these structures with that of a duplex DNA containing a thymine glycol at the same position as the abasic site and with that of a duplex DNA containing an abasic site in the middle of a curved DNA sequence offers some insight into the common and distinct structural features of damaged DNA sites. |
==About this Structure== | ==About this Structure== | ||
| - | 1A9H is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1A9H is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A9H OCA]. |
==Reference== | ==Reference== | ||
Structures of apurinic and apyrimidinic sites in duplex DNAs., Beger RD, Bolton PH, J Biol Chem. 1998 Jun 19;273(25):15565-73. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9624147 9624147] | Structures of apurinic and apyrimidinic sites in duplex DNAs., Beger RD, Bolton PH, J Biol Chem. 1998 Jun 19;273(25):15565-73. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9624147 9624147] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Beger, R | + | [[Category: Beger, R D.]] |
| - | [[Category: Bolton, P | + | [[Category: Bolton, P H.]] |
[[Category: apurinic dna]] | [[Category: apurinic dna]] | ||
[[Category: damaged dna]] | [[Category: damaged dna]] | ||
[[Category: water]] | [[Category: water]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:42:22 2008'' |
Revision as of 09:42, 21 February 2008
|
APURINIC DNA WITH BOUND WATER AT THE DAMAGED SITE AND O2 OF CYTOSINE, BETA FORM, NMR, 1 STRUCTURE
Overview
Natural and exogenous processes can give rise to abasic sites with either a purine or pyrimidine as the base on the opposing strand. The solution state structures of the apyrimidinic DNA duplex, with D6 indicating an abasic site, [sequence: see text] referred to as AD, and the apurinic DNA duplex with a dC17, referred to as CD, have been determined. A particularly striking difference is that the abasic site in CD is predominantly a beta hemiacetal, whereas in AD the alpha and beta forms are equally present. Hydrogen bonding with water by the abasic site and the base on the opposite strand appears to play a large role in determining the structure near the damaged site. Comparison of these structures with that of a duplex DNA containing a thymine glycol at the same position as the abasic site and with that of a duplex DNA containing an abasic site in the middle of a curved DNA sequence offers some insight into the common and distinct structural features of damaged DNA sites.
About this Structure
1A9H is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structures of apurinic and apyrimidinic sites in duplex DNAs., Beger RD, Bolton PH, J Biol Chem. 1998 Jun 19;273(25):15565-73. PMID:9624147
Page seeded by OCA on Thu Feb 21 11:42:22 2008
