1rfr
From Proteopedia
(New page: 200px<br /><applet load="1rfr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rfr" /> '''NMR structure of the 30mer stemloop-D of cox...) |
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'''NMR structure of the 30mer stemloop-D of coxsackieviral RNA'''<br /> | '''NMR structure of the 30mer stemloop-D of coxsackieviral RNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Stemloop D (SLD) of the 5' cloverleaf RNA is the cognate ligand of the | + | Stemloop D (SLD) of the 5' cloverleaf RNA is the cognate ligand of the coxsackievirus B3 (CVB3) 3C proteinase (3Cpro). Both are indispensable components of the viral replication initiation complex. SLD is a structurally autonomous subunit of the 5' cloverleaf. The SLD structure was solved by NMR spectroscopy to an rms deviation of 0.66 A (all heavy atoms). SLD contains a novel triple pyrimidine mismatch motif with a central Watson-Crick type C:U pair. SLD is capped by an apical uCACGg tetraloop adopting a structure highly similar to stable cUNCGg tetraloops. Binding of CVB3 3Cpro induces changes in NMR spectra for nucleotides adjacent to the triple pyrimidine mismatch and of the tetraloop implying them as sites of specific SLD:3Cpro interaction. The binding of 3Cpro to SLD requires the integrity of those structural elements, strongly suggesting that 3Cpro recognizes a structural motif instead of a specific sequence. |
==About this Structure== | ==About this Structure== | ||
| - | 1RFR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Human_coxsackievirus_b1 Human coxsackievirus b1]. Full crystallographic information is available from [http:// | + | 1RFR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Human_coxsackievirus_b1 Human coxsackievirus b1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RFR OCA]. |
==Reference== | ==Reference== | ||
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[[Category: loop with conformation similar to stable uncg-tetraloops and u:g closing base pair]] | [[Category: loop with conformation similar to stable uncg-tetraloops and u:g closing base pair]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:50:24 2008'' |
Revision as of 12:50, 21 February 2008
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NMR structure of the 30mer stemloop-D of coxsackieviral RNA
Overview
Stemloop D (SLD) of the 5' cloverleaf RNA is the cognate ligand of the coxsackievirus B3 (CVB3) 3C proteinase (3Cpro). Both are indispensable components of the viral replication initiation complex. SLD is a structurally autonomous subunit of the 5' cloverleaf. The SLD structure was solved by NMR spectroscopy to an rms deviation of 0.66 A (all heavy atoms). SLD contains a novel triple pyrimidine mismatch motif with a central Watson-Crick type C:U pair. SLD is capped by an apical uCACGg tetraloop adopting a structure highly similar to stable cUNCGg tetraloops. Binding of CVB3 3Cpro induces changes in NMR spectra for nucleotides adjacent to the triple pyrimidine mismatch and of the tetraloop implying them as sites of specific SLD:3Cpro interaction. The binding of 3Cpro to SLD requires the integrity of those structural elements, strongly suggesting that 3Cpro recognizes a structural motif instead of a specific sequence.
About this Structure
1RFR is a Protein complex structure of sequences from Human coxsackievirus b1. Full crystallographic information is available from OCA.
Reference
The structure of the stemloop D subdomain of coxsackievirus B3 cloverleaf RNA and its interaction with the proteinase 3C., Ohlenschlager O, Wohnert J, Bucci E, Seitz S, Hafner S, Ramachandran R, Zell R, Gorlach M, Structure. 2004 Feb;12(2):237-48. PMID:14962384
Page seeded by OCA on Thu Feb 21 14:50:24 2008
Categories: Human coxsackievirus b1 | Protein complex | Bucci, E. | Gorlach, M. | Hafner, S. | Ohlenschlager, O. | Ramachandran, R. | Seitz, S. | Wohnert, J. | Zell, R. | A-form helix stems | Base-paired u:u-c:u-u:u mismatch | Loop with conformation similar to stable uncg-tetraloops and u:g closing base pair
