1ess
From Proteopedia
(New page: 200px<br /><applet load="1ess" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ess" /> '''STEROID TETHERED DNA, NMR, MINIMIZED AVERAGE...) |
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| - | [[Image:1ess.gif|left|200px]]<br /><applet load="1ess" size=" | + | [[Image:1ess.gif|left|200px]]<br /><applet load="1ess" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''STEROID TETHERED DNA, NMR, MINIMIZED AVERAGE STRUCTURE'''<br /> | '''STEROID TETHERED DNA, NMR, MINIMIZED AVERAGE STRUCTURE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The solution structure of an estrone (Es)-tethered tandem DNA duplex | + | The solution structure of an estrone (Es)-tethered tandem DNA duplex consisting of two Es-tethered tetranucleotides and a target octameric DNA sequence is reported. The structure of this Es-tethered tandem duplex has been compared with a corresponding natural tandem duplex without estrones. The Tm of the 3'-Es-tethered tetranucleotide part of the tandem duplex increases by 5 degrees C, whereas the Tm of the 5'-Es-tethered tetranucleotide part increases by 7 degrees C, compared with the corresponding natural counterpart. The NMR structures of both the Es-tethered tandem duplex and the natural counterpart have been based on 24 experimental NMR constraints per residue. Despite the fact that there is considerable distortion at the junction of two Es-tethered tetranucleotides in the major groove of the Es-tethered DNA duplex compared to the natural counterpart, both duplexes do take up B-type DNA structures. It is likely that the spatial proximity of two Es residues, and the resulting hydrophobic interaction between them might be responsible for the increase of the thermal stability of the Es-tethered tandem duplex in comparison with the natural counterpart. |
==About this Structure== | ==About this Structure== | ||
| - | 1ESS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with ESO as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1ESS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=ESO:'>ESO</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESS OCA]. |
==Reference== | ==Reference== | ||
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[[Category: steroid tethered]] | [[Category: steroid tethered]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:31:08 2008'' |
Revision as of 10:31, 21 February 2008
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STEROID TETHERED DNA, NMR, MINIMIZED AVERAGE STRUCTURE
Overview
The solution structure of an estrone (Es)-tethered tandem DNA duplex consisting of two Es-tethered tetranucleotides and a target octameric DNA sequence is reported. The structure of this Es-tethered tandem duplex has been compared with a corresponding natural tandem duplex without estrones. The Tm of the 3'-Es-tethered tetranucleotide part of the tandem duplex increases by 5 degrees C, whereas the Tm of the 5'-Es-tethered tetranucleotide part increases by 7 degrees C, compared with the corresponding natural counterpart. The NMR structures of both the Es-tethered tandem duplex and the natural counterpart have been based on 24 experimental NMR constraints per residue. Despite the fact that there is considerable distortion at the junction of two Es-tethered tetranucleotides in the major groove of the Es-tethered DNA duplex compared to the natural counterpart, both duplexes do take up B-type DNA structures. It is likely that the spatial proximity of two Es residues, and the resulting hydrophobic interaction between them might be responsible for the increase of the thermal stability of the Es-tethered tandem duplex in comparison with the natural counterpart.
About this Structure
1ESS is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
The NMR structure of estrone (Es)-tethered tandem DNA duplex: [d(5'pCAGCp3')-Es] + [Es-d(5'pTCCA3')]: d(5'pTGGAGCTG3')., Denisov AY, Sandstrom A, Maltseva TV, Pyshnyi DV, Ivanova EM, Zarytova VF, Chattopadhyaya J, J Biomol Struct Dyn. 1997 Dec;15(3):499-516. PMID:9439997
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