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1na0
From Proteopedia
(New page: 200px<br /><applet load="1na0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1na0, resolution 1.60Å" /> '''Design of Stable alp...) |
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| - | [[Image:1na0.jpg|left|200px]]<br /><applet load="1na0" size=" | + | [[Image:1na0.jpg|left|200px]]<br /><applet load="1na0" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1na0, resolution 1.60Å" /> | caption="1na0, resolution 1.60Å" /> | ||
'''Design of Stable alpha-Helical Arrays from an Idealized TPR Motif'''<br /> | '''Design of Stable alpha-Helical Arrays from an Idealized TPR Motif'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The tetratricopeptide repeat (TPR) is a 34-amino acid alpha-helical motif | + | The tetratricopeptide repeat (TPR) is a 34-amino acid alpha-helical motif that occurs in over 300 different proteins. In the different proteins, three to sixteen or more TPR motifs occur in tandem arrays and function to mediate protein-protein interactions. The binding specificity of each TPR protein is different, although the underlying structural motif is the same. Here we describe a statistical approach to the design of an idealized TPR motif. We present the high-resolution X-ray crystal structures (to 1.55 and 1.6 A) of designed TPR proteins and describe their solution properties and stability. A detailed analysis of these structures provides an understanding of the TPR motif, how it is repeated to give helical arrays with different superhelical twists, and how a very stable framework may be constructed for future functional designs. |
==About this Structure== | ==About this Structure== | ||
| - | 1NA0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with ACT, PB, CL, NA, MG and IPT as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1NA0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=ACT:'>ACT</scene>, <scene name='pdbligand=PB:'>PB</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=IPT:'>IPT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NA0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: ]] | [[Category: ]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Andrea, L | + | [[Category: Andrea, L D.]] |
[[Category: Cocco, M.]] | [[Category: Cocco, M.]] | ||
[[Category: Main, E.]] | [[Category: Main, E.]] | ||
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[[Category: tpr]] | [[Category: tpr]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:03:50 2008'' |
Revision as of 12:03, 21 February 2008
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Design of Stable alpha-Helical Arrays from an Idealized TPR Motif
Overview
The tetratricopeptide repeat (TPR) is a 34-amino acid alpha-helical motif that occurs in over 300 different proteins. In the different proteins, three to sixteen or more TPR motifs occur in tandem arrays and function to mediate protein-protein interactions. The binding specificity of each TPR protein is different, although the underlying structural motif is the same. Here we describe a statistical approach to the design of an idealized TPR motif. We present the high-resolution X-ray crystal structures (to 1.55 and 1.6 A) of designed TPR proteins and describe their solution properties and stability. A detailed analysis of these structures provides an understanding of the TPR motif, how it is repeated to give helical arrays with different superhelical twists, and how a very stable framework may be constructed for future functional designs.
About this Structure
1NA0 is a Protein complex structure of sequences from [1] with , , , , and as ligands. Full crystallographic information is available from OCA.
Reference
Design of stable alpha-helical arrays from an idealized TPR motif., Main ER, Xiong Y, Cocco MJ, D'Andrea L, Regan L, Structure. 2003 May;11(5):497-508. PMID:12737816 [[Category: ]]
Page seeded by OCA on Thu Feb 21 14:03:50 2008
Categories: Protein complex | Andrea, L D. | Cocco, M. | Main, E. | Regan, L. | Xiong, Y. | ACT | CL | IPT | MG | NA | PB | Design | Tpr
