1wcm
From Proteopedia
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1WCM is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from | + | 1WCM is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]] with ZN and MG as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WCM OCA]]. |
==Reference== | ==Reference== | ||
Structures of complete RNA polymerase II and its subcomplex, Rpb4/7., Armache KJ, Mitterweger S, Meinhart A, Cramer P, J Biol Chem. 2005 Feb 25;280(8):7131-4. Epub 2004 Dec 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15591044 15591044] | Structures of complete RNA polymerase II and its subcomplex, Rpb4/7., Armache KJ, Mitterweger S, Meinhart A, Cramer P, J Biol Chem. 2005 Feb 25;280(8):7131-4. Epub 2004 Dec 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15591044 15591044] | ||
| + | [[Category: DNA-directed RNA polymerase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
| Line 34: | Line 35: | ||
[[Category: zinc-finger]] | [[Category: zinc-finger]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:19:13 2007'' |
Revision as of 08:14, 30 October 2007
|
COMPLETE 12-SUBUNIT RNA POLYMERASE II AT 3.8 ANG
Overview
We determined the x-ray structure of the RNA polymerase (Pol) II, subcomplex Rpb4/7 at 2.3 A resolution, combined it with a previous, structure of the 10-subunit polymerase core, and refined an atomic model, of the complete 12-subunit Pol II at 3.8-A resolution. Comparison of the, complete Pol II structure with structures of the Pol II core and free, Rpb4/7 shows that the core-Rpb4/7 interaction goes along with formation of, an alpha-helix in the linker region of the largest Pol II subunit and with, folding of the conserved Rpb7 tip loop. Details of the core-Rpb4/7, interface explain facilitated Rpb4/7 dissociation in a, temperature-sensitive Pol II mutant and specific assembly of Pol I with, its Rpb4/7 counterpart, A43/14. The refined atomic model of Pol II serves, as the new reference ... [(full description)]
About this Structure
1WCM is a [Protein complex] structure of sequences from [Saccharomyces cerevisiae] with ZN and MG as [ligands]. Active as [DNA-directed RNA polymerase], with EC number [2.7.7.6]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Structures of complete RNA polymerase II and its subcomplex, Rpb4/7., Armache KJ, Mitterweger S, Meinhart A, Cramer P, J Biol Chem. 2005 Feb 25;280(8):7131-4. Epub 2004 Dec 9. PMID:15591044
Page seeded by OCA on Tue Oct 30 10:19:13 2007
Categories: DNA-directed RNA polymerase | Protein complex | Saccharomyces cerevisiae | Armache, K.J. | Cramer, P. | Meinhart, A. | Mitterweger, S. | MG | ZN | Cellular rna polymerase | Dna-binding | Dna-dependent rna polymerase | Dna-directed rna polymerase | Metal-binding | Multisubunit co | Nuclear protein | Phosphorylation | Repeat | Transcription | Transferase | Zinc | Zinc-finger
