1aio
From Proteopedia
(New page: 200px<br /><applet load="1aio" size="450" color="white" frame="true" align="right" spinBox="true" caption="1aio, resolution 2.600Å" /> '''CRYSTAL STRUCTURE O...) |
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| - | [[Image:1aio.gif|left|200px]]<br /><applet load="1aio" size=" | + | [[Image:1aio.gif|left|200px]]<br /><applet load="1aio" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1aio, resolution 2.600Å" /> | caption="1aio, resolution 2.600Å" /> | ||
'''CRYSTAL STRUCTURE OF A DOUBLE-STRANDED DNA CONTAINING THE MAJOR ADDUCT OF THE ANTICANCER DRUG CISPLATIN'''<br /> | '''CRYSTAL STRUCTURE OF A DOUBLE-STRANDED DNA CONTAINING THE MAJOR ADDUCT OF THE ANTICANCER DRUG CISPLATIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The success of cisplatin in cancer chemotherapy derives from its ability | + | The success of cisplatin in cancer chemotherapy derives from its ability to crosslink DNA and alter the structure. Most cisplatin-DNA adducts are intrastrand d(GpG) and d(ApG) crosslinks, which unwind and bend the duplex to facilitate the binding of proteins that contain one or more high-mobility group (HMG) domains. When HMG-domain proteins such as HMG1, IXR (intrastrand-crosslink recognition) protein from yeast, or human upstream-binding factor (hUBF) bind cisplatin intrastrand crosslinks, they can be diverted from their natural binding sites on the genome and shield the adducts from excision repair. These activities sensitize cells to cisplatin and contribute to its cytotoxic properties. Crystallographic information about the structure of cisplatin-DNA adducts has been limited to short single-stranded deoxyoligonucleotides such as cis-[Pt(NH3)2(d(pGpG))]. Here we describe the X-ray structure at 2.6 A resolution of a double-stranded DNA dodecamer containing this adduct. Our information provides, to our knowledge, the first crystallographic look at a platinated DNA duplex and should help the design of new platinum and other metal crosslinking antitumour drug candidates. Moreover, the structure reveals a unique fusion of A- and B-type DNA segments that could be of more general importance. |
==About this Structure== | ==About this Structure== | ||
| - | 1AIO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with CPT as [http://en.wikipedia.org/wiki/ligand ligand]. This structure | + | 1AIO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CPT:'>CPT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. This structure supersedes the now removed PDB entry 1GPG. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AIO OCA]. |
==Reference== | ==Reference== | ||
Crystal structure of double-stranded DNA containing the major adduct of the anticancer drug cisplatin., Takahara PM, Rosenzweig AC, Frederick CA, Lippard SJ, Nature. 1995 Oct 19;377(6550):649-52. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7566180 7566180] | Crystal structure of double-stranded DNA containing the major adduct of the anticancer drug cisplatin., Takahara PM, Rosenzweig AC, Frederick CA, Lippard SJ, Nature. 1995 Oct 19;377(6550):649-52. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7566180 7566180] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Frederick, C | + | [[Category: Frederick, C A.]] |
| - | [[Category: Lippard, S | + | [[Category: Lippard, S J.]] |
| - | [[Category: Rosenzweig, A | + | [[Category: Rosenzweig, A C.]] |
| - | [[Category: Takahara, P | + | [[Category: Takahara, P M.]] |
[[Category: CPT]] | [[Category: CPT]] | ||
[[Category: complexed with drug]] | [[Category: complexed with drug]] | ||
| Line 22: | Line 22: | ||
[[Category: right handed dna]] | [[Category: right handed dna]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:44:57 2008'' |
Revision as of 09:44, 21 February 2008
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CRYSTAL STRUCTURE OF A DOUBLE-STRANDED DNA CONTAINING THE MAJOR ADDUCT OF THE ANTICANCER DRUG CISPLATIN
Overview
The success of cisplatin in cancer chemotherapy derives from its ability to crosslink DNA and alter the structure. Most cisplatin-DNA adducts are intrastrand d(GpG) and d(ApG) crosslinks, which unwind and bend the duplex to facilitate the binding of proteins that contain one or more high-mobility group (HMG) domains. When HMG-domain proteins such as HMG1, IXR (intrastrand-crosslink recognition) protein from yeast, or human upstream-binding factor (hUBF) bind cisplatin intrastrand crosslinks, they can be diverted from their natural binding sites on the genome and shield the adducts from excision repair. These activities sensitize cells to cisplatin and contribute to its cytotoxic properties. Crystallographic information about the structure of cisplatin-DNA adducts has been limited to short single-stranded deoxyoligonucleotides such as cis-[Pt(NH3)2(d(pGpG))]. Here we describe the X-ray structure at 2.6 A resolution of a double-stranded DNA dodecamer containing this adduct. Our information provides, to our knowledge, the first crystallographic look at a platinated DNA duplex and should help the design of new platinum and other metal crosslinking antitumour drug candidates. Moreover, the structure reveals a unique fusion of A- and B-type DNA segments that could be of more general importance.
About this Structure
1AIO is a Protein complex structure of sequences from [1] with as ligand. This structure supersedes the now removed PDB entry 1GPG. Full crystallographic information is available from OCA.
Reference
Crystal structure of double-stranded DNA containing the major adduct of the anticancer drug cisplatin., Takahara PM, Rosenzweig AC, Frederick CA, Lippard SJ, Nature. 1995 Oct 19;377(6550):649-52. PMID:7566180
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