1j4m
From Proteopedia
(New page: 200px<br /><applet load="1j4m" size="450" color="white" frame="true" align="right" spinBox="true" caption="1j4m" /> '''Minimized average structure of the 14-residu...) |
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'''Minimized average structure of the 14-residue peptide RG-KWTY-NG-ITYE-GR (MBH12)'''<br /> | '''Minimized average structure of the 14-residue peptide RG-KWTY-NG-ITYE-GR (MBH12)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Here we present a combinatorial approach to evolve a stable beta-hairpin | + | Here we present a combinatorial approach to evolve a stable beta-hairpin fold in a linear peptide. Starting with a de novo-designed linear peptide that shows a beta-hairpin structure population of around 30%, we selected four positions to build up a combinatorial library of 20(4) sequences. Deconvolution of the library using circular dichroism reduced such a sequence complexity to 36 defined sequences. Circular dichroism and NMR of these peptides resulted in the identification of two linear 14-aa-long peptides that in plain buffered solutions showed a percentage of beta-hairpin structure higher than 70%. Our results show how combinatorial approaches can be used to obtain highly structured peptide sequences that could be used as templates in which functionality can be introduced. |
==About this Structure== | ==About this Structure== | ||
| - | 1J4M is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1J4M is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J4M OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Lacroix, E.]] | [[Category: Lacroix, E.]] | ||
| - | [[Category: Pastor, M | + | [[Category: Pastor, M T.]] |
| - | [[Category: Paz, M | + | [[Category: Paz, M Lopez de la.]] |
[[Category: Perez-Paya, E.]] | [[Category: Perez-Paya, E.]] | ||
[[Category: Serrano, L.]] | [[Category: Serrano, L.]] | ||
[[Category: beta-hairpin]] | [[Category: beta-hairpin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:18:49 2008'' |
Revision as of 11:18, 21 February 2008
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Minimized average structure of the 14-residue peptide RG-KWTY-NG-ITYE-GR (MBH12)
Overview
Here we present a combinatorial approach to evolve a stable beta-hairpin fold in a linear peptide. Starting with a de novo-designed linear peptide that shows a beta-hairpin structure population of around 30%, we selected four positions to build up a combinatorial library of 20(4) sequences. Deconvolution of the library using circular dichroism reduced such a sequence complexity to 36 defined sequences. Circular dichroism and NMR of these peptides resulted in the identification of two linear 14-aa-long peptides that in plain buffered solutions showed a percentage of beta-hairpin structure higher than 70%. Our results show how combinatorial approaches can be used to obtain highly structured peptide sequences that could be used as templates in which functionality can be introduced.
About this Structure
1J4M is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Combinatorial approaches: a new tool to search for highly structured beta-hairpin peptides., Pastor MT, Lopez de la Paz M, Lacroix E, Serrano L, Perez-Paya E, Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):614-9. Epub 2002 Jan 8. PMID:11782528
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