1ngt
From Proteopedia
(New page: 200px<br /><applet load="1ngt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ngt, resolution 2.04Å" /> '''The Role of Minor Gr...) |
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| - | [[Image:1ngt.gif|left|200px]]<br /><applet load="1ngt" size=" | + | [[Image:1ngt.gif|left|200px]]<br /><applet load="1ngt" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ngt, resolution 2.04Å" /> | caption="1ngt, resolution 2.04Å" /> | ||
'''The Role of Minor Groove Functional Groups in DNA Hydration'''<br /> | '''The Role of Minor Groove Functional Groups in DNA Hydration'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Here we describe the crystal structure of modified [d(CGCGAATTCGCG)]2 | + | Here we describe the crystal structure of modified [d(CGCGAATTCGCG)]2 refined to 2.04 A. The modification, which affects only the two thymines at the central ApT step, involves isosteric removal of the 2-keto oxygen atoms and substitution of the N1 nitrogen with carbon. The crystal structure reveals the ability of this modified thymine to effectively base pair with adenine in [d(CGCGAAtTCGCG)]2. The structure also suggests that the minor groove 'spine of hydration' is destabilized but essentially intact. |
==About this Structure== | ==About this Structure== | ||
| - | 1NGT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1NGT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGT OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Lan, T.]] | [[Category: Lan, T.]] | ||
| - | [[Category: McLaughlin, L | + | [[Category: McLaughlin, L W.]] |
| - | [[Category: Williams, L | + | [[Category: Williams, L D.]] |
| - | [[Category: Woods, K | + | [[Category: Woods, K K.]] |
[[Category: MG]] | [[Category: MG]] | ||
[[Category: dna conformation]] | [[Category: dna conformation]] | ||
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[[Category: spine of hydration]] | [[Category: spine of hydration]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:05:56 2008'' |
Revision as of 12:05, 21 February 2008
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The Role of Minor Groove Functional Groups in DNA Hydration
Overview
Here we describe the crystal structure of modified [d(CGCGAATTCGCG)]2 refined to 2.04 A. The modification, which affects only the two thymines at the central ApT step, involves isosteric removal of the 2-keto oxygen atoms and substitution of the N1 nitrogen with carbon. The crystal structure reveals the ability of this modified thymine to effectively base pair with adenine in [d(CGCGAAtTCGCG)]2. The structure also suggests that the minor groove 'spine of hydration' is destabilized but essentially intact.
About this Structure
1NGT is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
The role of minor groove functional groups in DNA hydration., Woods KK, Lan T, McLaughlin LW, Williams LD, Nucleic Acids Res. 2003 Mar 1;31(5):1536-40. PMID:12595562
Page seeded by OCA on Thu Feb 21 14:05:56 2008
