1vs2
From Proteopedia
(New page: 200px<br /><applet load="1vs2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1vs2, resolution 2.000Å" /> '''Interactions of qui...) |
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| - | [[Image:1vs2.gif|left|200px]]<br /><applet load="1vs2" size=" | + | [[Image:1vs2.gif|left|200px]]<br /><applet load="1vs2" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1vs2, resolution 2.000Å" /> | caption="1vs2, resolution 2.000Å" /> | ||
'''Interactions of quinoxaline antibiotic and DNA: the molecular structure of a TRIOSTIN A-D(GCGTACGC) complex'''<br /> | '''Interactions of quinoxaline antibiotic and DNA: the molecular structure of a TRIOSTIN A-D(GCGTACGC) complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of a DNA octamer d(GCGTACGC) complexed to an | + | The crystal structure of a DNA octamer d(GCGTACGC) complexed to an antitumor antibiotic, triostin A, has been solved and refined to 2.2 A resolution by x-ray diffraction analysis. The antibiotic molecule acts as a true bis intercalator surrounding the d(CpG) sequence at either end of the unwound right-handed DNA double helix. As previously observed in the structure of triostin A-d(CGTACG) complex (A.H.-J. Wang, et. al., Science, 225, 1115-1121 (1984)), the alanine amino acid residues of the drug molecule form sequence-specific hydrogen bonds to guanines in the minor groove. The two central A.T base pairs are in Hoogsteen configuration with adenine in the syn conformation. In addition, the two terminal G.C base pairs flanking the quinoxaline rings are also held together by Hoogsteen base pairing. This is the first observation in an oligonucleotide of. Hoogsteen G.C base pairs where the cytosine is protonated. The principal functional components of a bis-intercalative compound are discussed. |
==About this Structure== | ==About this Structure== | ||
| - | 1VS2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with QUI, NCY, MVA, SER and ALA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1VS2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=QUI:'>QUI</scene>, <scene name='pdbligand=NCY:'>NCY</scene>, <scene name='pdbligand=MVA:'>MVA</scene>, <scene name='pdbligand=SER:'>SER</scene> and <scene name='pdbligand=ALA:'>ALA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VS2 OCA]. |
==Reference== | ==Reference== | ||
Interactions of quinoxaline antibiotic and DNA: the molecular structure of a triostin A-d(GCGTACGC) complex., Wang AH, Ughetto G, Quigley GJ, Rich A, J Biomol Struct Dyn. 1986 Dec;4(3):319-42. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=3271447 3271447] | Interactions of quinoxaline antibiotic and DNA: the molecular structure of a triostin A-d(GCGTACGC) complex., Wang AH, Ughetto G, Quigley GJ, Rich A, J Biomol Struct Dyn. 1986 Dec;4(3):319-42. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=3271447 3271447] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Quigley, G | + | [[Category: Quigley, G J.]] |
[[Category: Rich, A.]] | [[Category: Rich, A.]] | ||
[[Category: Ughetto, G.]] | [[Category: Ughetto, G.]] | ||
| - | [[Category: Wang, A | + | [[Category: Wang, A H.J.]] |
[[Category: ALA]] | [[Category: ALA]] | ||
[[Category: MVA]] | [[Category: MVA]] | ||
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[[Category: right handed dna]] | [[Category: right handed dna]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:38:02 2008'' |
Revision as of 13:38, 21 February 2008
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Interactions of quinoxaline antibiotic and DNA: the molecular structure of a TRIOSTIN A-D(GCGTACGC) complex
Overview
The crystal structure of a DNA octamer d(GCGTACGC) complexed to an antitumor antibiotic, triostin A, has been solved and refined to 2.2 A resolution by x-ray diffraction analysis. The antibiotic molecule acts as a true bis intercalator surrounding the d(CpG) sequence at either end of the unwound right-handed DNA double helix. As previously observed in the structure of triostin A-d(CGTACG) complex (A.H.-J. Wang, et. al., Science, 225, 1115-1121 (1984)), the alanine amino acid residues of the drug molecule form sequence-specific hydrogen bonds to guanines in the minor groove. The two central A.T base pairs are in Hoogsteen configuration with adenine in the syn conformation. In addition, the two terminal G.C base pairs flanking the quinoxaline rings are also held together by Hoogsteen base pairing. This is the first observation in an oligonucleotide of. Hoogsteen G.C base pairs where the cytosine is protonated. The principal functional components of a bis-intercalative compound are discussed.
About this Structure
1VS2 is a Protein complex structure of sequences from [1] with , , , and as ligands. Full crystallographic information is available from OCA.
Reference
Interactions of quinoxaline antibiotic and DNA: the molecular structure of a triostin A-d(GCGTACGC) complex., Wang AH, Ughetto G, Quigley GJ, Rich A, J Biomol Struct Dyn. 1986 Dec;4(3):319-42. PMID:3271447
Page seeded by OCA on Thu Feb 21 15:38:02 2008
Categories: Protein complex | Quigley, G J. | Rich, A. | Ughetto, G. | Wang, A H.J. | ALA | MVA | NCY | QUI | SER | Complexed with drug | Double helix | Right handed dna
