1rrv
From Proteopedia
(New page: 200px<br /><applet load="1rrv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rrv, resolution 2.0Å" /> '''X-ray crystal structu...) |
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| - | [[Image:1rrv.gif|left|200px]]<br /><applet load="1rrv" size=" | + | [[Image:1rrv.gif|left|200px]]<br /><applet load="1rrv" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1rrv, resolution 2.0Å" /> | caption="1rrv, resolution 2.0Å" /> | ||
'''X-ray crystal structure of TDP-vancosaminyltransferase GtfD as a complex with TDP and the natural substrate, desvancosaminyl vancomycin.'''<br /> | '''X-ray crystal structure of TDP-vancosaminyltransferase GtfD as a complex with TDP and the natural substrate, desvancosaminyl vancomycin.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The TDP-vancosaminyltransferase GtfD catalyzes the attachment of | + | The TDP-vancosaminyltransferase GtfD catalyzes the attachment of L-vancosamine to a monoglucosylated heptapeptide intermediate during the final stage of vancomycin biosynthesis. Glycosyltransferases from this and similar antibiotic pathways are potential tools for the design of new compounds that are effective against vancomycin resistant bacterial strains. We have determined the X-ray crystal structure of GtfD as a complex with TDP and the natural glycopeptide substrate at 2.0 A resolution. GtfD, a member of the bidomain GT-B glycosyltransferase superfamily, binds TDP in the interdomain cleft, while the aglycone acceptor binds in a deep crevice in the N-terminal domain. However, the two domains are more interdependent in terms of substrate binding and overall structure than was evident in the structures of closely related glycosyltransferases GtfA and GtfB. Structural and kinetic analyses support the identification of Asp13 as a catalytic general base, with a possible secondary role for Thr10. Several residues have also been identified as being involved in donor sugar binding and recognition. |
==About this Structure== | ==About this Structure== | ||
| - | 1RRV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Amycolatopsis_orientalis Amycolatopsis orientalis] with K, DVV, TYD and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1RRV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Amycolatopsis_orientalis Amycolatopsis orientalis] with <scene name='pdbligand=K:'>K</scene>, <scene name='pdbligand=DVV:'>DVV</scene>, <scene name='pdbligand=TYD:'>TYD</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RRV OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Amycolatopsis orientalis]] | [[Category: Amycolatopsis orientalis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Garavito, R | + | [[Category: Garavito, R M.]] |
| - | [[Category: Losey, H | + | [[Category: Losey, H C.]] |
[[Category: Lu, W.]] | [[Category: Lu, W.]] | ||
| - | [[Category: Mulichak, A | + | [[Category: Mulichak, A M.]] |
| - | [[Category: Walsh, C | + | [[Category: Walsh, C T.]] |
[[Category: DVV]] | [[Category: DVV]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
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[[Category: rossmann fold]] | [[Category: rossmann fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:53:54 2008'' |
Revision as of 12:53, 21 February 2008
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X-ray crystal structure of TDP-vancosaminyltransferase GtfD as a complex with TDP and the natural substrate, desvancosaminyl vancomycin.
Overview
The TDP-vancosaminyltransferase GtfD catalyzes the attachment of L-vancosamine to a monoglucosylated heptapeptide intermediate during the final stage of vancomycin biosynthesis. Glycosyltransferases from this and similar antibiotic pathways are potential tools for the design of new compounds that are effective against vancomycin resistant bacterial strains. We have determined the X-ray crystal structure of GtfD as a complex with TDP and the natural glycopeptide substrate at 2.0 A resolution. GtfD, a member of the bidomain GT-B glycosyltransferase superfamily, binds TDP in the interdomain cleft, while the aglycone acceptor binds in a deep crevice in the N-terminal domain. However, the two domains are more interdependent in terms of substrate binding and overall structure than was evident in the structures of closely related glycosyltransferases GtfA and GtfB. Structural and kinetic analyses support the identification of Asp13 as a catalytic general base, with a possible secondary role for Thr10. Several residues have also been identified as being involved in donor sugar binding and recognition.
About this Structure
1RRV is a Single protein structure of sequence from Amycolatopsis orientalis with , , and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of vancosaminyltransferase GtfD from the vancomycin biosynthetic pathway: interactions with acceptor and nucleotide ligands., Mulichak AM, Lu W, Losey HC, Walsh CT, Garavito RM, Biochemistry. 2004 May 11;43(18):5170-80. PMID:15122882
Page seeded by OCA on Thu Feb 21 14:53:54 2008
Categories: Amycolatopsis orientalis | Single protein | Garavito, R M. | Losey, H C. | Lu, W. | Mulichak, A M. | Walsh, C T. | DVV | GOL | K | TYD | Glycosyltransferase | Gt-b | Rossmann fold
