1njo
From Proteopedia
(New page: 200px<br /><applet load="1njo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1njo, resolution 3.70Å" /> '''The crystal structur...) |
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| - | [[Image:1njo.gif|left|200px]]<br /><applet load="1njo" size=" | + | [[Image:1njo.gif|left|200px]]<br /><applet load="1njo" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1njo, resolution 3.70Å" /> | caption="1njo, resolution 3.70Å" /> | ||
'''The crystal structure of the 50S Large ribosomal subunit from Deinococcus radiodurans complexed with a short substrate analog ACCPuromycin (ACCP)'''<br /> | '''The crystal structure of the 50S Large ribosomal subunit from Deinococcus radiodurans complexed with a short substrate analog ACCPuromycin (ACCP)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Crystal structures of tRNA mimics complexed with the large ribosomal | + | Crystal structures of tRNA mimics complexed with the large ribosomal subunit of Deinococcus radiodurans indicate that remote interactions determine the precise orientation of tRNA in the peptidyl-transferase center (PTC). The PTC tolerates various orientations of puromycin derivatives and its flexibility allows the conformational rearrangements required for peptide-bond formation. Sparsomycin binds to A2602 and alters the PTC conformation. H69, the intersubunit-bridge connecting the PTC and decoding site, may also participate in tRNA placement and translocation. A spiral rotation of the 3' end of the A-site tRNA around a 2-fold axis of symmetry identified within the PTC suggests a unified ribosomal machinery for peptide-bond formation, A-to-P-site translocation, and entrance of nascent proteins into the exit tunnel. Similar 2-fold related regions, detected in all known structures of large ribosomal subunits, indicate the universality of this mechanism. |
==About this Structure== | ==About this Structure== | ||
| - | 1NJO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http:// | + | 1NJO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NJO OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Bashan, A.]] | [[Category: Bashan, A.]] | ||
[[Category: Berisio, R.]] | [[Category: Berisio, R.]] | ||
| - | [[Category: Hansen, H | + | [[Category: Hansen, H A.]] |
| - | [[Category: Harms, J | + | [[Category: Harms, J M.]] |
[[Category: Schluenzen, F.]] | [[Category: Schluenzen, F.]] | ||
[[Category: Yonath, A.]] | [[Category: Yonath, A.]] | ||
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[[Category: trna]] | [[Category: trna]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:06:50 2008'' |
Revision as of 12:06, 21 February 2008
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The crystal structure of the 50S Large ribosomal subunit from Deinococcus radiodurans complexed with a short substrate analog ACCPuromycin (ACCP)
Overview
Crystal structures of tRNA mimics complexed with the large ribosomal subunit of Deinococcus radiodurans indicate that remote interactions determine the precise orientation of tRNA in the peptidyl-transferase center (PTC). The PTC tolerates various orientations of puromycin derivatives and its flexibility allows the conformational rearrangements required for peptide-bond formation. Sparsomycin binds to A2602 and alters the PTC conformation. H69, the intersubunit-bridge connecting the PTC and decoding site, may also participate in tRNA placement and translocation. A spiral rotation of the 3' end of the A-site tRNA around a 2-fold axis of symmetry identified within the PTC suggests a unified ribosomal machinery for peptide-bond formation, A-to-P-site translocation, and entrance of nascent proteins into the exit tunnel. Similar 2-fold related regions, detected in all known structures of large ribosomal subunits, indicate the universality of this mechanism.
About this Structure
1NJO is a Single protein structure of sequence from Deinococcus radiodurans. Full crystallographic information is available from OCA.
Reference
Structural basis of the ribosomal machinery for peptide bond formation, translocation, and nascent chain progression., Bashan A, Agmon I, Zarivach R, Schluenzen F, Harms J, Berisio R, Bartels H, Franceschi F, Auerbach T, Hansen HA, Kossoy E, Kessler M, Yonath A, Mol Cell. 2003 Jan;11(1):91-102. PMID:12535524
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