1jbf
From Proteopedia
(New page: 200px<br /><applet load="1jbf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jbf" /> '''Hairpin Peptide that Inhibits IgE Activity b...) |
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| - | [[Image:1jbf.gif|left|200px]]<br /><applet load="1jbf" size=" | + | [[Image:1jbf.gif|left|200px]]<br /><applet load="1jbf" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1jbf" /> | caption="1jbf" /> | ||
'''Hairpin Peptide that Inhibits IgE Activity by Binding to the High Affinity IgE Receptor'''<br /> | '''Hairpin Peptide that Inhibits IgE Activity by Binding to the High Affinity IgE Receptor'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A family of structured peptides that bind to FcepsilonRIalpha, the | + | A family of structured peptides that bind to FcepsilonRIalpha, the alpha-chain of the high-affinity receptor for IgE, has been identified. Binding selections using FcepsilonRIalpha and polyvalent peptide-phage libraries yielded a dominant 18-residue peptide-phage clone, as well as related sequences that did not resemble fragments of IgE. Synthetic peptides based on these sequences inhibited IgE binding to its receptor, and were found by NMR analysis to adopt a stable beta-hairpin structure in solution. Optimized peptides with micromolar receptor affinity exhibited high stability in biological fluids and inhibited cellular histamine release in an in vitro bioassay of IgE activity. The structure-activity relationships of these peptides, which are less than 1% of the size of IgE, suggest an overlap between their binding site and that of IgE on FcepsilonRI. Thus, the peptides demonstrate that blocking a small epitope on this receptor chain is sufficient to block IgE activity. Such structured peptides represent a possible starting point for the design of novel antagonists, and offer the potential for testing in vivo a new approach for treating allergic disease. |
==About this Structure== | ==About this Structure== | ||
| - | 1JBF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with ACE as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1JBF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=ACE:'>ACE</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JBF OCA]. |
==Reference== | ==Reference== | ||
A novel family of hairpin peptides that inhibit IgE activity by binding to the high-affinity IgE receptor., Nakamura GR, Starovasnik MA, Reynolds ME, Lowman HB, Biochemistry. 2001 Aug 21;40(33):9828-35. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11502176 11502176] | A novel family of hairpin peptides that inhibit IgE activity by binding to the high-affinity IgE receptor., Nakamura GR, Starovasnik MA, Reynolds ME, Lowman HB, Biochemistry. 2001 Aug 21;40(33):9828-35. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11502176 11502176] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Lowman, H | + | [[Category: Lowman, H B.]] |
| - | [[Category: Nakamura, G | + | [[Category: Nakamura, G R.]] |
| - | [[Category: Reynolds, M | + | [[Category: Reynolds, M E.]] |
| - | [[Category: Starovasnik, M | + | [[Category: Starovasnik, M A.]] |
[[Category: ACE]] | [[Category: ACE]] | ||
[[Category: beta-hairpin]] | [[Category: beta-hairpin]] | ||
[[Category: type i turn]] | [[Category: type i turn]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:20:43 2008'' |
Revision as of 11:20, 21 February 2008
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Hairpin Peptide that Inhibits IgE Activity by Binding to the High Affinity IgE Receptor
Overview
A family of structured peptides that bind to FcepsilonRIalpha, the alpha-chain of the high-affinity receptor for IgE, has been identified. Binding selections using FcepsilonRIalpha and polyvalent peptide-phage libraries yielded a dominant 18-residue peptide-phage clone, as well as related sequences that did not resemble fragments of IgE. Synthetic peptides based on these sequences inhibited IgE binding to its receptor, and were found by NMR analysis to adopt a stable beta-hairpin structure in solution. Optimized peptides with micromolar receptor affinity exhibited high stability in biological fluids and inhibited cellular histamine release in an in vitro bioassay of IgE activity. The structure-activity relationships of these peptides, which are less than 1% of the size of IgE, suggest an overlap between their binding site and that of IgE on FcepsilonRI. Thus, the peptides demonstrate that blocking a small epitope on this receptor chain is sufficient to block IgE activity. Such structured peptides represent a possible starting point for the design of novel antagonists, and offer the potential for testing in vivo a new approach for treating allergic disease.
About this Structure
1JBF is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
A novel family of hairpin peptides that inhibit IgE activity by binding to the high-affinity IgE receptor., Nakamura GR, Starovasnik MA, Reynolds ME, Lowman HB, Biochemistry. 2001 Aug 21;40(33):9828-35. PMID:11502176
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