1s23
From Proteopedia
(New page: 200px<br /><applet load="1s23" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s23, resolution 1.60Å" /> '''Crystal Structure An...) |
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| - | [[Image:1s23.gif|left|200px]]<br /><applet load="1s23" size=" | + | [[Image:1s23.gif|left|200px]]<br /><applet load="1s23" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1s23, resolution 1.60Å" /> | caption="1s23, resolution 1.60Å" /> | ||
'''Crystal Structure Analysis of the B-DNA Decamer CGCAATTGCG'''<br /> | '''Crystal Structure Analysis of the B-DNA Decamer CGCAATTGCG'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The deoxyoligonucleotide d(CGCAATTGCG) has previously been crystallized in | + | The deoxyoligonucleotide d(CGCAATTGCG) has previously been crystallized in four different space groups. The crystals diffract to moderate resolution (2.3-2.9 A). Here, a fifth crystal form that diffracts to higher resolution (1.6 A) is presented which was obtained thanks to the use of Co2+ and cryogenic temperatures. The availability of five different crystal structures allows a thorough analysis of the conformational variability of this DNA sequence. It is concluded that the central hexamer sequence CAATTG has a practically constant conformation under all conditions, whilst the terminal base pairs at both ends vary considerably as a result of differing interactions in the crystals. The new crystal structure presented here is stabilized by guanine-Co2+-guanine interactions and the formation of C1+ -G8.C3 triplexes between neighbouring duplexes. As a result of the higher resolution of the crystal structure, a more regular structure was obtained and a clear definition of the spine of hydration was observed which was not visible in the four previous structures. |
==About this Structure== | ==About this Structure== | ||
| - | 1S23 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with CO as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1S23 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CO:'>CO</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S23 OCA]. |
==Reference== | ==Reference== | ||
DNA variability in five crystal structures of d(CGCAATTGCG)., Valls N, Wright G, Steiner RA, Murshudov GN, Subirana JA, Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):680-5. Epub 2004, Mar 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15039556 15039556] | DNA variability in five crystal structures of d(CGCAATTGCG)., Valls N, Wright G, Steiner RA, Murshudov GN, Subirana JA, Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):680-5. Epub 2004, Mar 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15039556 15039556] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Murshudov, G | + | [[Category: Murshudov, G N.]] |
| - | [[Category: Steiner, R | + | [[Category: Steiner, R A.]] |
| - | [[Category: Subirana, J | + | [[Category: Subirana, J A.]] |
[[Category: Valls, N.]] | [[Category: Valls, N.]] | ||
[[Category: Wright, G.]] | [[Category: Wright, G.]] | ||
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[[Category: variability]] | [[Category: variability]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:57:07 2008'' |
Revision as of 12:57, 21 February 2008
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Crystal Structure Analysis of the B-DNA Decamer CGCAATTGCG
Overview
The deoxyoligonucleotide d(CGCAATTGCG) has previously been crystallized in four different space groups. The crystals diffract to moderate resolution (2.3-2.9 A). Here, a fifth crystal form that diffracts to higher resolution (1.6 A) is presented which was obtained thanks to the use of Co2+ and cryogenic temperatures. The availability of five different crystal structures allows a thorough analysis of the conformational variability of this DNA sequence. It is concluded that the central hexamer sequence CAATTG has a practically constant conformation under all conditions, whilst the terminal base pairs at both ends vary considerably as a result of differing interactions in the crystals. The new crystal structure presented here is stabilized by guanine-Co2+-guanine interactions and the formation of C1+ -G8.C3 triplexes between neighbouring duplexes. As a result of the higher resolution of the crystal structure, a more regular structure was obtained and a clear definition of the spine of hydration was observed which was not visible in the four previous structures.
About this Structure
1S23 is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
DNA variability in five crystal structures of d(CGCAATTGCG)., Valls N, Wright G, Steiner RA, Murshudov GN, Subirana JA, Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):680-5. Epub 2004, Mar 23. PMID:15039556
Page seeded by OCA on Thu Feb 21 14:57:07 2008
