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3tf4
From Proteopedia
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==About this Structure== | ==About this Structure== | ||
| - | 3TF4 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/ | + | 3TF4 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca]] with GLC and CA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Cellulase Cellulase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4]]. Structure known Active Sites: AA1, AB1, CA1, CA2, CA3 and CA4. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3TF4 OCA]]. |
==Reference== | ==Reference== | ||
Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca., Sakon J, Irwin D, Wilson DB, Karplus PA, Nat Struct Biol. 1997 Oct;4(10):810-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9334746 9334746] | Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca., Sakon J, Irwin D, Wilson DB, Karplus PA, Nat Struct Biol. 1997 Oct;4(10):810-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9334746 9334746] | ||
| + | [[Category: Cellulase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: | + | [[Category: Thermobifida fusca]] |
[[Category: Karplus, P.A.]] | [[Category: Karplus, P.A.]] | ||
[[Category: Sakon, J.]] | [[Category: Sakon, J.]] | ||
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[[Category: glycosyl hydrolase]] | [[Category: glycosyl hydrolase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:25:28 2007'' |
Revision as of 08:20, 30 October 2007
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ENDO/EXOCELLULASE:CELLOTRIOSE FROM THERMOMONOSPORA
Overview
Cellulase E4 from Thermomonospora fusca is unusual in that it has, characteristics of both exo- and endo-cellulases. Here we report the, crystal structure of a 68K M(r) fragment of E4 (E4-68) at 1.9 A, resolution. E4-68 contains both a family 9 catalytic domain, exhibiting an, (alpha/alpha)6 barrel fold, and a family III cellulose binding domain, having an antiparallel beta-sandwich fold. While neither of these folds is, novel, E4-68 provides the first cellulase structure having interacting, catalytic and cellulose binding domains. The complexes of E4-68 with, cellopentaose, cellotriose and cellobiose reveal conformational changes, associated with ligand binding and allow us to propose a catalytic, mechanism for family 9 enzymes. We also provide evidence that E4 has two, novel ... [(full description)]
About this Structure
3TF4 is a [Single protein] structure of sequence from [Thermobifida fusca] with GLC and CA as [ligands]. Active as [Cellulase], with EC number [3.2.1.4]. Structure known Active Sites: AA1, AB1, CA1, CA2, CA3 and CA4. Full crystallographic information is available from [OCA].
Reference
Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca., Sakon J, Irwin D, Wilson DB, Karplus PA, Nat Struct Biol. 1997 Oct;4(10):810-8. PMID:9334746
Page seeded by OCA on Tue Oct 30 10:25:28 2007
