1s2r
From Proteopedia
(New page: 200px<br /><applet load="1s2r" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s2r, resolution 1.53Å" /> '''A High Resolution Cr...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1s2r.gif|left|200px]]<br /><applet load="1s2r" size=" | + | [[Image:1s2r.gif|left|200px]]<br /><applet load="1s2r" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1s2r, resolution 1.53Å" /> | caption="1s2r, resolution 1.53Å" /> | ||
'''A High Resolution Crystal Structure of [d(CGCAAATTTGCG)]2'''<br /> | '''A High Resolution Crystal Structure of [d(CGCAAATTTGCG)]2'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of [d(CGCAAATTTGCG)]2 has been determined to 1.5 | + | The crystal structure of [d(CGCAAATTTGCG)]2 has been determined to 1.5 A resolution, representing the first high-resolution structure of this DNA fragment. The ion interactions are novel. A spermine molecule replaces a Mg2+ observed in analogous structures. Unlike lower-resolution structures, the minor groove is narrow and the major groove lacks extra Watson-Crick hydrogen bonds. In addition, a monolayer of solvent sites, including a "spine of hydration", is visible in the minor groove. The crystal of [d(CGCAAATTTGCG)]2 was grown from a solution containing spermine, magnesium, and lithium. The conformation recapitulates that of "monovalent-minus" DNA. |
==About this Structure== | ==About this Structure== | ||
| - | 1S2R is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with SPM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1S2R is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=SPM:'>SPM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S2R OCA]. |
==Reference== | ==Reference== | ||
High-resolution structure of an extended A-tract: [d(CGCAAATTTGCG)]2., Woods KK, Maehigashi T, Howerton SB, Sines CC, Tannenbaum S, Williams LD, J Am Chem Soc. 2004 Dec 1;126(47):15330-1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15563130 15563130] | High-resolution structure of an extended A-tract: [d(CGCAAATTTGCG)]2., Woods KK, Maehigashi T, Howerton SB, Sines CC, Tannenbaum S, Williams LD, J Am Chem Soc. 2004 Dec 1;126(47):15330-1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15563130 15563130] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Howerton, S | + | [[Category: Howerton, S B.]] |
[[Category: Maehigashi, T.]] | [[Category: Maehigashi, T.]] | ||
[[Category: Tannenbaum, S.]] | [[Category: Tannenbaum, S.]] | ||
| - | [[Category: Williams, L | + | [[Category: Williams, L D.]] |
| - | [[Category: Woods, K | + | [[Category: Woods, K K.]] |
[[Category: SPM]] | [[Category: SPM]] | ||
[[Category: dodecamer]] | [[Category: dodecamer]] | ||
[[Category: minor groove hydration]] | [[Category: minor groove hydration]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:57:16 2008'' |
Revision as of 12:57, 21 February 2008
|
A High Resolution Crystal Structure of [d(CGCAAATTTGCG)]2
Overview
The crystal structure of [d(CGCAAATTTGCG)]2 has been determined to 1.5 A resolution, representing the first high-resolution structure of this DNA fragment. The ion interactions are novel. A spermine molecule replaces a Mg2+ observed in analogous structures. Unlike lower-resolution structures, the minor groove is narrow and the major groove lacks extra Watson-Crick hydrogen bonds. In addition, a monolayer of solvent sites, including a "spine of hydration", is visible in the minor groove. The crystal of [d(CGCAAATTTGCG)]2 was grown from a solution containing spermine, magnesium, and lithium. The conformation recapitulates that of "monovalent-minus" DNA.
About this Structure
1S2R is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
High-resolution structure of an extended A-tract: [d(CGCAAATTTGCG)]2., Woods KK, Maehigashi T, Howerton SB, Sines CC, Tannenbaum S, Williams LD, J Am Chem Soc. 2004 Dec 1;126(47):15330-1. PMID:15563130
Page seeded by OCA on Thu Feb 21 14:57:16 2008
