1jm1
From Proteopedia
(New page: 200px<br /><applet load="1jm1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jm1, resolution 1.11Å" /> '''Crystal structure of...) |
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| - | [[Image:1jm1.jpg|left|200px]]<br /><applet load="1jm1" size=" | + | [[Image:1jm1.jpg|left|200px]]<br /><applet load="1jm1" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1jm1, resolution 1.11Å" /> | caption="1jm1, resolution 1.11Å" /> | ||
'''Crystal structure of the soluble domain of the Rieske protein II (soxF) from Sulfolobus acidocaldarius'''<br /> | '''Crystal structure of the soluble domain of the Rieske protein II (soxF) from Sulfolobus acidocaldarius'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The first crystal structure of an archaeal Rieske iron-sulfur protein, the | + | The first crystal structure of an archaeal Rieske iron-sulfur protein, the soluble domain of Rieske iron-sulfur protein II (soxF) from the hyperthermo-acidophile Sulfolobus acidocaldarius, has been solved by multiple wavelength anomalous dispersion (MAD) and has been refined to 1.1 A resolution. SoxF is a subunit of the terminal oxidase supercomplex SoxM in the plasma membrane of S. acidocaldarius that combines features of a cytochrome bc(1) complex and a cytochrome c oxidase. The [2Fe-2S] cluster of soxF is most likely the primary electron acceptor during the oxidation of caldariella quinone by the cytochrome a(587)/Rieske subcomplex. The geometry of the [2Fe-2S] cluster and the structure of the cluster-binding site are almost identical in soxF and the Rieske proteins from eucaryal cytochrome bc(1) and b(6)f complexes, suggesting a strict conservation of the catalytic mechanism. The main domain of soxF and part of the cluster-binding domain, though structurally related, show a significantly divergent structure with respect to topology, non-covalent interactions and surface charges. The divergent structure of soxF reflects a different topology of the soxM complex compared to eucaryal bc complexes and the adaptation of the protein to the extreme ambient conditions on the outer membrane surface of a hyperthermo-acidophilic organism. |
==About this Structure== | ==About this Structure== | ||
| - | 1JM1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius] with MG and FES as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1JM1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=FES:'>FES</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JM1 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Ladenstein, R.]] | [[Category: Ladenstein, R.]] | ||
[[Category: Schaefer, G.]] | [[Category: Schaefer, G.]] | ||
| - | [[Category: Schmidt, C | + | [[Category: Schmidt, C L.]] |
[[Category: FES]] | [[Category: FES]] | ||
[[Category: MG]] | [[Category: MG]] | ||
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[[Category: rieske iron-sulfur protein]] | [[Category: rieske iron-sulfur protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:24:14 2008'' |
Revision as of 11:24, 21 February 2008
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Crystal structure of the soluble domain of the Rieske protein II (soxF) from Sulfolobus acidocaldarius
Overview
The first crystal structure of an archaeal Rieske iron-sulfur protein, the soluble domain of Rieske iron-sulfur protein II (soxF) from the hyperthermo-acidophile Sulfolobus acidocaldarius, has been solved by multiple wavelength anomalous dispersion (MAD) and has been refined to 1.1 A resolution. SoxF is a subunit of the terminal oxidase supercomplex SoxM in the plasma membrane of S. acidocaldarius that combines features of a cytochrome bc(1) complex and a cytochrome c oxidase. The [2Fe-2S] cluster of soxF is most likely the primary electron acceptor during the oxidation of caldariella quinone by the cytochrome a(587)/Rieske subcomplex. The geometry of the [2Fe-2S] cluster and the structure of the cluster-binding site are almost identical in soxF and the Rieske proteins from eucaryal cytochrome bc(1) and b(6)f complexes, suggesting a strict conservation of the catalytic mechanism. The main domain of soxF and part of the cluster-binding domain, though structurally related, show a significantly divergent structure with respect to topology, non-covalent interactions and surface charges. The divergent structure of soxF reflects a different topology of the soxM complex compared to eucaryal bc complexes and the adaptation of the protein to the extreme ambient conditions on the outer membrane surface of a hyperthermo-acidophilic organism.
About this Structure
1JM1 is a Single protein structure of sequence from Sulfolobus acidocaldarius with and as ligands. Full crystallographic information is available from OCA.
Reference
The structure of the soluble domain of an archaeal Rieske iron-sulfur protein at 1.1 A resolution., Bonisch H, Schmidt CL, Schafer G, Ladenstein R, J Mol Biol. 2002 Jun 7;319(3):791-805. PMID:12054871
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