C-di-GMP specific phosphodiesterases

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Revision as of 15:09, 15 July 2009 (edit) Tilman Schirmer (Talk | contribs) (New page: back == YukI == <applet load='2w27.pdb' scene='User:Tilman_Schirmer/Sandbox_202/Yuki_dimer/3' size='300' frame='true' align='right' caption='YukI 2w27' /> <sc...) ← Previous diff		Revision as of 07:57, 16 May 2011 (edit) (undo) Michal Harel (Talk | contribs) Next diff →
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		+	==3D structure of YukI==
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		+	[[2bas]] – BsYkuI – ''Bacillus subtilis''<br />
		+	[[2w27]] – BsYkuI + C-di-GMP
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	==References==		==References==

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Revision as of 07:57, 16 May 2011

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Contents 1 YukI 2 BlrP1 3 3D structure of YukI 4 References

YukI

spinqualitylabelsall models YukI 2w27 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

is a dimer with each protomer composed of an (with TIM barrel fold), a long , and a .

The is situated at the C-terminal end of the central β-barrel of the EAL domain. The residues of the active site are well conserved, including E33 and L35 of the EAL signature motif.

The is bound flat upon the actve site with the divalent metal (here Ca⁺⁺) being sandwiched between binding site and c-di-GMP.

BlrP1

spinqualitylabelsall models BlrP1 3gg0 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

3D structure of YukI

2bas – BsYkuI – Bacillus subtilis
2w27 – BsYkuI + C-di-GMP

References

YkuI structure 2w27:

• Minasov G, Padavattan S, Shuvalova L, Brunzelle JS, Miller DJ, Basle A, Massa C, Collart FR, Schirmer T, Anderson WF. Crystal structures of YkuI and its complex with second messenger cyclic Di-GMP suggest catalytic mechanism of phosphodiester bond cleavage by EAL domains. J Biol Chem. 2009 May 8;284(19):13174-84. Epub 2009 Feb 24. PMID:19244251 doi:10.1074/jbc.M808221200

BlrP1 structure 3gg0:

• Barends TR, Hartmann E, Griese JJ, Beitlich T, Kirienko NV, Ryjenkov DA, Reinstein J, Shoeman RL, Gomelsky M, Schlichting I. Structure and mechanism of a bacterial light-regulated cyclic nucleotide phosphodiesterase. Nature. 2009 Jun 18;459(7249):1015-8. PMID:19536266 doi:10.1038/nature07966