1nyd
From Proteopedia
(New page: 200px<br /><applet load="1nyd" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nyd" /> '''Solution structure of DNA quadruplex GCGGTGG...) |
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'''Solution structure of DNA quadruplex GCGGTGGAT'''<br /> | '''Solution structure of DNA quadruplex GCGGTGGAT'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure formed by the DNA sequence d(GCGGTGGAT) in a 100 mM Na(+) | + | The structure formed by the DNA sequence d(GCGGTGGAT) in a 100 mM Na(+) solution has been determined using molecular dynamics calculations constrained by distance and dihedral restraints derived from NMR experiments performed at isotopic natural abundance. The sequence folds into a dimer of dimers. Each symmetry-related half contains two parallel stranded G:G:G:G tetrads flanked by an A:A mismatch and by four-stranded G:C:G:C tetrads. Each of the two juxtaposed G:C:G:C tetrads is composed of alternating antiparallel strands from the two halves of the dimer. For each single strand, a thymine intersperses a double chain reversal connecting the juxtaposed G:G:G:G tetrads. This architecture has potential implications in genetic recombination. It suggests a pathway for oligomerization involving association of quadruplex entities through GpC steps. |
==About this Structure== | ==About this Structure== | ||
| - | 1NYD is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1NYD is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NYD OCA]. |
==Reference== | ==Reference== | ||
Association of DNA quadruplexes through G:C:G:C tetrads. Solution structure of d(GCGGTGGAT)., Webba da Silva M, Biochemistry. 2003 Dec 16;42(49):14356-65. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14661946 14661946] | Association of DNA quadruplexes through G:C:G:C tetrads. Solution structure of d(GCGGTGGAT)., Webba da Silva M, Biochemistry. 2003 Dec 16;42(49):14356-65. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14661946 14661946] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Silva, M | + | [[Category: Silva, M Webba da.]] |
[[Category: dna]] | [[Category: dna]] | ||
[[Category: double chain reversal]] | [[Category: double chain reversal]] | ||
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[[Category: quadruplex]] | [[Category: quadruplex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:11:22 2008'' |
Revision as of 12:11, 21 February 2008
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Solution structure of DNA quadruplex GCGGTGGAT
Overview
The structure formed by the DNA sequence d(GCGGTGGAT) in a 100 mM Na(+) solution has been determined using molecular dynamics calculations constrained by distance and dihedral restraints derived from NMR experiments performed at isotopic natural abundance. The sequence folds into a dimer of dimers. Each symmetry-related half contains two parallel stranded G:G:G:G tetrads flanked by an A:A mismatch and by four-stranded G:C:G:C tetrads. Each of the two juxtaposed G:C:G:C tetrads is composed of alternating antiparallel strands from the two halves of the dimer. For each single strand, a thymine intersperses a double chain reversal connecting the juxtaposed G:G:G:G tetrads. This architecture has potential implications in genetic recombination. It suggests a pathway for oligomerization involving association of quadruplex entities through GpC steps.
About this Structure
1NYD is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Association of DNA quadruplexes through G:C:G:C tetrads. Solution structure of d(GCGGTGGAT)., Webba da Silva M, Biochemistry. 2003 Dec 16;42(49):14356-65. PMID:14661946
Page seeded by OCA on Thu Feb 21 14:11:22 2008
