1fml
From Proteopedia
(New page: 200px<br /><applet load="1fml" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fml, resolution 2.75Å" /> '''CRYSTAL STRUCTURE OF...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1fml.gif|left|200px]]<br /><applet load="1fml" size=" | + | [[Image:1fml.gif|left|200px]]<br /><applet load="1fml" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1fml, resolution 2.75Å" /> | caption="1fml, resolution 2.75Å" /> | ||
'''CRYSTAL STRUCTURE OF RETINOL DEHYDRATASE IN A COMPLEX WITH RETINOL AND PAP'''<br /> | '''CRYSTAL STRUCTURE OF RETINOL DEHYDRATASE IN A COMPLEX WITH RETINOL AND PAP'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We report here the crystal structure of retinol dehydratase, an enzyme | + | We report here the crystal structure of retinol dehydratase, an enzyme that catalyzes the synthesis of anhydroretinol. The enzyme is a member of the sulfotransferase superfamily and its crystal structure reveals the insertion of a helical lid into a canonical sulfotransferase fold. Site-directed mutations demonstrate that this inserted lid is necessary for anhydroretinol production but not for sulfonation; thus, insertion of a helical lid can convert a sulfotransferase into a dehydratase. |
==About this Structure== | ==About this Structure== | ||
| - | 1FML is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Spodoptera_frugiperda Spodoptera frugiperda] with CA, A3P and RTL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1FML is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Spodoptera_frugiperda Spodoptera frugiperda] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=A3P:'>A3P</scene> and <scene name='pdbligand=RTL:'>RTL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FML OCA]. |
==Reference== | ==Reference== | ||
| Line 15: | Line 15: | ||
[[Category: Buck, J.]] | [[Category: Buck, J.]] | ||
[[Category: Kobayashi, M.]] | [[Category: Kobayashi, M.]] | ||
| - | [[Category: Newcomer, M | + | [[Category: Newcomer, M E.]] |
[[Category: Pakhomova, S.]] | [[Category: Pakhomova, S.]] | ||
[[Category: A3P]] | [[Category: A3P]] | ||
| Line 26: | Line 26: | ||
[[Category: sulfotransferase]] | [[Category: sulfotransferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:40:16 2008'' |
Revision as of 10:40, 21 February 2008
|
CRYSTAL STRUCTURE OF RETINOL DEHYDRATASE IN A COMPLEX WITH RETINOL AND PAP
Overview
We report here the crystal structure of retinol dehydratase, an enzyme that catalyzes the synthesis of anhydroretinol. The enzyme is a member of the sulfotransferase superfamily and its crystal structure reveals the insertion of a helical lid into a canonical sulfotransferase fold. Site-directed mutations demonstrate that this inserted lid is necessary for anhydroretinol production but not for sulfonation; thus, insertion of a helical lid can convert a sulfotransferase into a dehydratase.
About this Structure
1FML is a Single protein structure of sequence from Spodoptera frugiperda with , and as ligands. Full crystallographic information is available from OCA.
Reference
A helical lid converts a sulfotransferase to a dehydratase., Pakhomova S, Kobayashi M, Buck J, Newcomer ME, Nat Struct Biol. 2001 May;8(5):447-51. PMID:11323722
Page seeded by OCA on Thu Feb 21 12:40:16 2008
Categories: Single protein | Spodoptera frugiperda | Buck, J. | Kobayashi, M. | Newcomer, M E. | Pakhomova, S. | A3P | CA | RTL | 5'-diphosphate | Adenosine 3' | Dehydratase | Retinol | Sulfotransferase
