1nzg
From Proteopedia
(New page: 200px<br /><applet load="1nzg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nzg, resolution 1.60Å" /> '''Crystal structure of...) |
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| - | [[Image:1nzg.gif|left|200px]]<br /><applet load="1nzg" size=" | + | [[Image:1nzg.gif|left|200px]]<br /><applet load="1nzg" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1nzg, resolution 1.60Å" /> | caption="1nzg, resolution 1.60Å" /> | ||
'''Crystal structure of A-DNA decamer GCGTA(3ME)ACGC, with a modified 5-methyluridine'''<br /> | '''Crystal structure of A-DNA decamer GCGTA(3ME)ACGC, with a modified 5-methyluridine'''<br /> | ||
==Overview== | ==Overview== | ||
| - | [structure: see text] Oligonucleotides with a novel, 2'-O-[2-[2-(N,N-dimethylamino)ethoxy]ethyl] (2'-O-DMAEOE) modification | + | [structure: see text] Oligonucleotides with a novel, 2'-O-[2-[2-(N,N-dimethylamino)ethoxy]ethyl] (2'-O-DMAEOE) modification have been synthesized. This modification, a cationic analogue of the 2'-O-(2-methoxyethyl) (2'-O-MOE) modification, exhibits high binding affinity to target RNA (but not to DNA) and exceptional resistance to nuclease degradation. Analysis of the crystal structure of a self-complementary oligonucleotide containing a single 2'-O-DMAEOE modification explains the importance of charge factors and gauche effects on the observed antisense properties. 2'-O-DMAEOE modified oligonucleotides are ideal candidates for antisense drugs. |
==About this Structure== | ==About this Structure== | ||
| - | 1NZG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1NZG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NZG OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Manoharan, M.]] | [[Category: Manoharan, M.]] | ||
[[Category: Minasov, G.]] | [[Category: Minasov, G.]] | ||
| - | [[Category: Prakash, T | + | [[Category: Prakash, T P.]] |
[[Category: Prhavc, M.]] | [[Category: Prhavc, M.]] | ||
[[Category: 2'-modified oligonucleotides]] | [[Category: 2'-modified oligonucleotides]] | ||
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[[Category: crystal structure]] | [[Category: crystal structure]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:11:43 2008'' |
Revision as of 12:11, 21 February 2008
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Crystal structure of A-DNA decamer GCGTA(3ME)ACGC, with a modified 5-methyluridine
Overview
[structure: see text] Oligonucleotides with a novel, 2'-O-[2-[2-(N,N-dimethylamino)ethoxy]ethyl] (2'-O-DMAEOE) modification have been synthesized. This modification, a cationic analogue of the 2'-O-(2-methoxyethyl) (2'-O-MOE) modification, exhibits high binding affinity to target RNA (but not to DNA) and exceptional resistance to nuclease degradation. Analysis of the crystal structure of a self-complementary oligonucleotide containing a single 2'-O-DMAEOE modification explains the importance of charge factors and gauche effects on the observed antisense properties. 2'-O-DMAEOE modified oligonucleotides are ideal candidates for antisense drugs.
About this Structure
1NZG is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
2'-O-[2-[2-(N,N-dimethylamino)ethoxy]ethyl] modified oligonucleotides: symbiosis of charge interaction factors and stereoelectronic effects., Prhavc M, Prakash TP, Minasov G, Cook PD, Egli M, Manoharan M, Org Lett. 2003 Jun 12;5(12):2017-20. PMID:12790517
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