1fnn
From Proteopedia
(New page: 200px<br /><applet load="1fnn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fnn, resolution 2.0Å" /> '''CRYSTAL STRUCTURE OF ...) |
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| - | [[Image:1fnn.gif|left|200px]]<br /><applet load="1fnn" size=" | + | [[Image:1fnn.gif|left|200px]]<br /><applet load="1fnn" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1fnn, resolution 2.0Å" /> | caption="1fnn, resolution 2.0Å" /> | ||
'''CRYSTAL STRUCTURE OF CDC6P FROM PYROBACULUM AEROPHILUM'''<br /> | '''CRYSTAL STRUCTURE OF CDC6P FROM PYROBACULUM AEROPHILUM'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Cdc6/Cdc18 is a conserved and essential component of prereplication | + | Cdc6/Cdc18 is a conserved and essential component of prereplication complexes. The 2.0 A crystal structure of an archaeal Cdc6 ortholog, in conjunction with a mutational analysis of the homologous Cdc18 protein from Schizosaccharomyces pombe, reveals novel aspects of Cdc6/Cdc18 function. Two domains of Cdc6 form an AAA+-type nucleotide binding fold that is observed bound to Mg.ADP. A third domain adopts a winged-helix fold similar to known DNA binding modules. Sequence comparisons show that the winged-helix domain is conserved in Orc1, and mutagenesis data demonstrate that this region of Cdc6/Cdc18 is required for function in vivo. Additional mutational analyses suggest that nucleotide binding and/or hydrolysis by Cdc6/Cdc18 is required not only for progression through S phase, but also for maintenance of checkpoint control during S phase. |
==About this Structure== | ==About this Structure== | ||
| - | 1FNN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrobaculum_aerophilum Pyrobaculum aerophilum] with MG and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1FNN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrobaculum_aerophilum Pyrobaculum aerophilum] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FNN OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Pyrobaculum aerophilum]] | [[Category: Pyrobaculum aerophilum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Berger, J | + | [[Category: Berger, J M.]] |
[[Category: DeAngelis, K.]] | [[Category: DeAngelis, K.]] | ||
[[Category: DeRyckere, D.]] | [[Category: DeRyckere, D.]] | ||
[[Category: Liu, J.]] | [[Category: Liu, J.]] | ||
| - | [[Category: Martin, G | + | [[Category: Martin, G S.]] |
| - | [[Category: Smith, C | + | [[Category: Smith, C L.]] |
[[Category: ADP]] | [[Category: ADP]] | ||
[[Category: MG]] | [[Category: MG]] | ||
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[[Category: orc1]] | [[Category: orc1]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:40:36 2008'' |
Revision as of 10:40, 21 February 2008
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CRYSTAL STRUCTURE OF CDC6P FROM PYROBACULUM AEROPHILUM
Overview
Cdc6/Cdc18 is a conserved and essential component of prereplication complexes. The 2.0 A crystal structure of an archaeal Cdc6 ortholog, in conjunction with a mutational analysis of the homologous Cdc18 protein from Schizosaccharomyces pombe, reveals novel aspects of Cdc6/Cdc18 function. Two domains of Cdc6 form an AAA+-type nucleotide binding fold that is observed bound to Mg.ADP. A third domain adopts a winged-helix fold similar to known DNA binding modules. Sequence comparisons show that the winged-helix domain is conserved in Orc1, and mutagenesis data demonstrate that this region of Cdc6/Cdc18 is required for function in vivo. Additional mutational analyses suggest that nucleotide binding and/or hydrolysis by Cdc6/Cdc18 is required not only for progression through S phase, but also for maintenance of checkpoint control during S phase.
About this Structure
1FNN is a Single protein structure of sequence from Pyrobaculum aerophilum with and as ligands. Full crystallographic information is available from OCA.
Reference
Structure and function of Cdc6/Cdc18: implications for origin recognition and checkpoint control., Liu J, Smith CL, DeRyckere D, DeAngelis K, Martin GS, Berger JM, Mol Cell. 2000 Sep;6(3):637-48. PMID:11030343
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