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1s9s
From Proteopedia
(New page: 200px<br /><applet load="1s9s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s9s" /> '''SOLUTION STRUCTURE OF MLV PSI SITE'''<br /> ...) |
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| - | [[Image:1s9s.gif|left|200px]]<br /><applet load="1s9s" size=" | + | [[Image:1s9s.gif|left|200px]]<br /><applet load="1s9s" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1s9s" /> | caption="1s9s" /> | ||
'''SOLUTION STRUCTURE OF MLV PSI SITE'''<br /> | '''SOLUTION STRUCTURE OF MLV PSI SITE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The full length, positive-strand genome of the Moloney Murine Leukemia | + | The full length, positive-strand genome of the Moloney Murine Leukemia Virus contains a "core encapsidation signal" that is essential for efficient genome packaging during virus assembly. We have determined the structure of a 101-nucleotide RNA that contains this signal (called mPsi) using a novel isotope-edited NMR approach. The method is robust and should be generally applicable to larger RNAs. mPsi folds into three stem loops, two of which (SL-C and SL-D) co-stack to form an extended helix. The third stem loop (SL-B) is connected to SL-C by a flexible, four-nucleotide linker. The structure contains five mismatched base-pairs, an unusual C.CG base-triple platform, and a novel "A-minor K-turn," in which unpaired adenosine bases A340 and A341 of a GGAA bulge pack in the minor groove of a proximal stem, and a bulged distal uridine (U319) forms a hydrogen bond with the phosphodiester of A341. Phylogenetic analyses indicate that these essential structural elements are conserved among the murine C-type retroviruses. |
==About this Structure== | ==About this Structure== | ||
| - | 1S9S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1S9S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S9S OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Dey, A.]] | [[Category: Dey, A.]] | ||
[[Category: Habib, D.]] | [[Category: Habib, D.]] | ||
| - | [[Category: Souza, V | + | [[Category: Souza, V D.]] |
| - | [[Category: Summers, M | + | [[Category: Summers, M F.]] |
[[Category: a-c mismatch]] | [[Category: a-c mismatch]] | ||
[[Category: a-minor k-turn]] | [[Category: a-minor k-turn]] | ||
| Line 25: | Line 25: | ||
[[Category: u-u mismatch]] | [[Category: u-u mismatch]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:59:23 2008'' |
Revision as of 12:59, 21 February 2008
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SOLUTION STRUCTURE OF MLV PSI SITE
Overview
The full length, positive-strand genome of the Moloney Murine Leukemia Virus contains a "core encapsidation signal" that is essential for efficient genome packaging during virus assembly. We have determined the structure of a 101-nucleotide RNA that contains this signal (called mPsi) using a novel isotope-edited NMR approach. The method is robust and should be generally applicable to larger RNAs. mPsi folds into three stem loops, two of which (SL-C and SL-D) co-stack to form an extended helix. The third stem loop (SL-B) is connected to SL-C by a flexible, four-nucleotide linker. The structure contains five mismatched base-pairs, an unusual C.CG base-triple platform, and a novel "A-minor K-turn," in which unpaired adenosine bases A340 and A341 of a GGAA bulge pack in the minor groove of a proximal stem, and a bulged distal uridine (U319) forms a hydrogen bond with the phosphodiester of A341. Phylogenetic analyses indicate that these essential structural elements are conserved among the murine C-type retroviruses.
About this Structure
1S9S is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
NMR structure of the 101-nucleotide core encapsidation signal of the Moloney murine leukemia virus., D'Souza V, Dey A, Habib D, Summers MF, J Mol Biol. 2004 Mar 19;337(2):427-42. PMID:15003457
Page seeded by OCA on Thu Feb 21 14:59:23 2008
Categories: Protein complex | Dey, A. | Habib, D. | Souza, V D. | Summers, M F. | A-c mismatch | A-minor k-turn | Bulge | G-a mismatch | G-u mismatch | Mlv | Stem loop | U-u mismatch
