1saq
From Proteopedia
(New page: 200px<br /><applet load="1saq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1saq, resolution 2.7Å" /> '''Crystal Structure of ...) |
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| - | [[Image:1saq.gif|left|200px]]<br /><applet load="1saq" size=" | + | [[Image:1saq.gif|left|200px]]<br /><applet load="1saq" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1saq, resolution 2.7Å" /> | caption="1saq, resolution 2.7Å" /> | ||
'''Crystal Structure of the RNA octamer GIC(GA)GCC'''<br /> | '''Crystal Structure of the RNA octamer GIC(GA)GCC'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structures of two RNA octamers, 5'-GGC(GA)GCC-3' and | + | The crystal structures of two RNA octamers, 5'-GGC(GA)GCC-3' and 5'-GIC(GA)GCC-3', have been determined from X-ray diffraction data to 2.8 and 2.7 A resolution, respectively. The RNA octamers crystallize in isomorphous unit cells containing two mispairs arranged in a self-complementary manner and one single strand in the asymmetric unit. The single strand pairs with another single strand related by crystallographic symmetry to form a third unique double helix. Tandem non-Watson-Crick G.A/A.G base pairs of the sheared type comprise an internal loop in the middle of each duplex. The NMR structure of this octameric RNA sequence is also known, allowing comparison of the variation between the six crystallographic duplexes and the solution structure. In the symmetric duplex of the octamer containing inosine, the sheared G.A pairs incorporate a bound water molecule. This duplex also binds one water molecule per strand in the minor groove adjacent to the G.A pairs. |
==About this Structure== | ==About this Structure== | ||
| - | 1SAQ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1SAQ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SAQ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Baeyens, K.]] | [[Category: Baeyens, K.]] | ||
| - | [[Category: Holbrook, S | + | [[Category: Holbrook, S R.]] |
| - | [[Category: Jang, S | + | [[Category: Jang, S B.]] |
| - | [[Category: Jeong, M | + | [[Category: Jeong, M S.]] |
| - | [[Category: Jr, J | + | [[Category: Jr, J SantaLucia.]] |
[[Category: Turner, D.]] | [[Category: Turner, D.]] | ||
[[Category: rna double helix]] | [[Category: rna double helix]] | ||
[[Category: rna tandem g-a base pair]] | [[Category: rna tandem g-a base pair]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:59:32 2008'' |
Revision as of 12:59, 21 February 2008
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Crystal Structure of the RNA octamer GIC(GA)GCC
Overview
The crystal structures of two RNA octamers, 5'-GGC(GA)GCC-3' and 5'-GIC(GA)GCC-3', have been determined from X-ray diffraction data to 2.8 and 2.7 A resolution, respectively. The RNA octamers crystallize in isomorphous unit cells containing two mispairs arranged in a self-complementary manner and one single strand in the asymmetric unit. The single strand pairs with another single strand related by crystallographic symmetry to form a third unique double helix. Tandem non-Watson-Crick G.A/A.G base pairs of the sheared type comprise an internal loop in the middle of each duplex. The NMR structure of this octameric RNA sequence is also known, allowing comparison of the variation between the six crystallographic duplexes and the solution structure. In the symmetric duplex of the octamer containing inosine, the sheared G.A pairs incorporate a bound water molecule. This duplex also binds one water molecule per strand in the minor groove adjacent to the G.A pairs.
About this Structure
1SAQ is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structures of two RNA octamers containing tandem G.A base pairs., Jang SB, Baeyens K, Jeong MS, SantaLucia J Jr, Turner D, Holbrook SR, Acta Crystallogr D Biol Crystallogr. 2004 May;60(Pt 5):829-35. Epub 2004, Apr 21. PMID:15103128
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