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1wmw
From Proteopedia
(New page: 200px<br /><applet load="1wmw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wmw, resolution 1.55Å" /> '''Crystal structure of...) |
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| - | [[Image:1wmw.gif|left|200px]]<br /><applet load="1wmw" size=" | + | [[Image:1wmw.gif|left|200px]]<br /><applet load="1wmw" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1wmw, resolution 1.55Å" /> | caption="1wmw, resolution 1.55Å" /> | ||
'''Crystal structure of geranulgeranyl diphosphate synthase from Thermus thermophilus'''<br /> | '''Crystal structure of geranulgeranyl diphosphate synthase from Thermus thermophilus'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Geranylgeranyl diphosphate (GGPP) synthase from Thermus thermophilus HB8 | + | Geranylgeranyl diphosphate (GGPP) synthase from Thermus thermophilus HB8 was expressed in Escherichia coli, purified to homogeneity and crystallized both as the recombinant native protein and its selenomethionine (SeMet) derivative. Well diffracting crystals of these proteins were obtained belonging to the tetragonal space group P4(1) or P4(3), with unit-cell parameters a = b = 139.88, c = 73.37 A. There were two homodimers in the asymmetric unit. A native data set was collected to 1.55 A resolution and a data set suitable for MAD phasing was collected to 2.40 A resolution on beamline BL40B2 at SPring-8. |
==About this Structure== | ==About this Structure== | ||
| - | 1WMW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http:// | + | 1WMW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WMW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Nishio, K.]] | [[Category: Nishio, K.]] | ||
[[Category: Nodake, Y.]] | [[Category: Nodake, Y.]] | ||
| - | [[Category: RSGI, RIKEN | + | [[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]] |
[[Category: Suto, K.]] | [[Category: Suto, K.]] | ||
[[Category: ggpp]] | [[Category: ggpp]] | ||
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[[Category: thermus]] | [[Category: thermus]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:46:05 2008'' |
Revision as of 13:46, 21 February 2008
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Crystal structure of geranulgeranyl diphosphate synthase from Thermus thermophilus
Overview
Geranylgeranyl diphosphate (GGPP) synthase from Thermus thermophilus HB8 was expressed in Escherichia coli, purified to homogeneity and crystallized both as the recombinant native protein and its selenomethionine (SeMet) derivative. Well diffracting crystals of these proteins were obtained belonging to the tetragonal space group P4(1) or P4(3), with unit-cell parameters a = b = 139.88, c = 73.37 A. There were two homodimers in the asymmetric unit. A native data set was collected to 1.55 A resolution and a data set suitable for MAD phasing was collected to 2.40 A resolution on beamline BL40B2 at SPring-8.
About this Structure
1WMW is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
Expression, purification, crystallization and preliminary X-ray studies of geranylgeranyl diphosphate synthase from Thermus thermophilus HB8., Nishio K, Nodake Y, Hamada K, Suto K, Nakagawa N, Kuramitsu S, Miura K, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):178-80. Epub 2003, Dec 18. PMID:14684922
Page seeded by OCA on Thu Feb 21 15:46:05 2008
Categories: Single protein | Thermus thermophilus | Hamada, K. | Kawamoto, M. | Kuramitu, S. | Miura, K. | Nakagawa, N. | Nishio, K. | Nodake, Y. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Suto, K. | Ggpp | Prenyl diphosphate synthase | Riken structural genomics/proteomics initiative | Rsgi | Structural genomics | Thermus
